Detailed information of TA system TA059046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2198675..2199204
• Replicon: chromosome
• Accession: NZ_CP013621
• Organism: Staphylococcus aureus strain RIVM3897

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AUC50_RS11045
• Protein ID: WP_000621175.1
• Coordinates: 2198675..2199037 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: AUC50_RS11050
• Protein ID: WP_000948331.1
• Coordinates: 2199034..2199204 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AUC50_RS11020	2195653..2196423	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
AUC50_RS11025	2196398..2196877	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
AUC50_RS11030	2196879..2197205	-	327	WP_001052491.1	anti-sigma factor antagonist	-
AUC50_RS11035	2197324..2198325	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
AUC50_RS11045	2198675..2199037	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AUC50_RS11050	2199034..2199204	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
AUC50_RS11055	2199289..2200437	-	1149	WP_001281139.1	alanine racemase	-
AUC50_RS11060	2200503..2200862	-	360	WP_000581197.1	holo-ACP synthase	-
AUC50_RS11065	2200866..2201357	-	492	WP_001286980.1	PH domain-containing protein	-
AUC50_RS11070	2201350..2202927	-	1578	WP_001294650.1	PH domain-containing protein	-
AUC50_RS11075	2202920..2203399	-	480	WP_001287081.1	hypothetical protein	-
AUC50_RS11080	2203608..2204168	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T59046 WP_000621175.1 NZ_CP013621:c2199037-2198675 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T59046 NZ_CP013621:c2199037-2198675 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT59046 WP_000948331.1 NZ_CP013621:c2199204-2199034 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT59046 NZ_CP013621:c2199204-2199034 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1