Detailed information of TA system TA059014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 337961..338658
• Replicon: plasmid pRspN731e
• Accession: NZ_CP013606
• Organism: Rhizobium sp. N731

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AMK02_RS29020
• Protein ID: WP_064808526.1
• Coordinates: 337961..338407 (-)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AMK02_RS29025
• Protein ID: WP_064808528.1
• Coordinates: 338404..338658 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK02_RS29000	333763..335055	+	1293	WP_064808514.1	L-rhamnose catabolism isomerase	-
AMK02_RS29005	335123..335581	-	459	WP_064808516.1	carboxymuconolactone decarboxylase family protein	-
AMK02_RS29010	335690..336559	+	870	WP_064808518.1	sigma-70 family RNA polymerase sigma factor	-
AMK02_RS29015	336730..337938	+	1209	WP_064808853.1	amidohydrolase/deacetylase family metallohydrolase	-
AMK02_RS29020	337961..338407	-	447	WP_064808526.1	type II toxin-antitoxin system VapC family toxin	Toxin
AMK02_RS29025	338404..338658	-	255	WP_064808528.1	hypothetical protein	Antitoxin
AMK02_RS29030	338746..339585	-	840	WP_064808530.1	IclR family transcriptional regulator	-
AMK02_RS29035	339739..340161	-	423	WP_064808532.1	type II toxin-antitoxin system VapC family toxin	-
AMK02_RS29040	340161..340409	-	249	WP_064808552.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AMK02_RS29045	340524..341381	+	858	WP_064808855.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..506687	506687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15799.01 Da
• Isoelectric Point: 4.8781

>T59014 WP_064808526.1 NZ_CP013606:c338407-337961 [Rhizobium sp. N731]
MIVLDTNVLCEAMKPAPNPAVRSWLNEQVAETLYLSSVTLAELLFGIGTLPDGRRKAALSETLGGVLDLFGDRILPFDVG
AARHYADLAVRARADGKGFPTPNGYIAAIAASKGFMIATRDVSPFEAAGLIVINPWNHRSSEASQEDS

Nucleotide

• Length: 447 bp

>T59014 NZ_CP013606:c338407-337961 [Rhizobium sp. N731]
ATGATCGTCCTGGATACGAACGTCCTTTGCGAGGCGATGAAACCGGCTCCTAATCCGGCAGTGCGTTCGTGGCTGAACGA
GCAGGTCGCTGAAACCTTGTATCTATCGAGTGTGACCTTGGCCGAACTGCTGTTTGGCATCGGTACGTTGCCGGACGGTC
GGCGCAAAGCGGCTCTGAGCGAAACGCTCGGCGGCGTGCTCGACCTGTTTGGCGATCGGATATTGCCGTTCGACGTCGGA
GCGGCCCGGCATTATGCCGACCTTGCCGTCAGAGCGCGAGCGGACGGCAAAGGCTTCCCAACGCCCAATGGATATATCGC
CGCCATTGCCGCTTCGAAGGGCTTTATGATCGCAACGCGCGACGTGAGCCCGTTTGAGGCGGCAGGACTGATTGTCATAA
ATCCCTGGAACCATCGATCATCTGAGGCCTCTCAGGAGGATTCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9385.62 Da
• Isoelectric Point: 5.1358

>AT59014 WP_064808528.1 NZ_CP013606:c338658-338404 [Rhizobium sp. N731]
MAVLTVRNVPDEVHRALRVRAAMHGRSTEAEVREILESAVKPEQRIRMGDALAALSRQQGLTDDDFAVFDQVRDSAPAEP
LKFE

Nucleotide

• Length: 255 bp

>AT59014 NZ_CP013606:c338658-338404 [Rhizobium sp. N731]
ATGGCCGTTCTGACAGTTCGAAATGTGCCCGATGAAGTGCATCGGGCATTGCGCGTGCGGGCGGCCATGCACGGCCGCAG
CACCGAGGCGGAAGTTCGTGAAATCCTGGAAAGTGCGGTGAAGCCGGAGCAACGCATTCGTATGGGGGATGCCTTGGCGG
CGCTCAGTCGGCAGCAGGGGCTGACCGATGATGATTTCGCAGTGTTCGATCAGGTACGCGACAGCGCGCCGGCGGAGCCG
TTGAAGTTTGAATGA