Detailed information of TA system TA059013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 175400..176166
• Replicon: plasmid pRspN731e
• Accession: NZ_CP013606
• Organism: Rhizobium sp. N731

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: AMK02_RS28250
• Protein ID: WP_064808266.1
• Coordinates: 175684..176166 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: AMK02_RS28245
• Protein ID: WP_064808264.1
• Coordinates: 175400..175678 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK02_RS31575	171326..171508	-	183	WP_123876252.1	hypothetical protein	-
AMK02_RS30940	171613..171786	-	174	Protein_150	tautomerase family protein	-
AMK02_RS28220	172480..172989	+	510	WP_064808258.1	hypothetical protein	-
AMK02_RS28225	173235..173582	+	348	WP_064808260.1	helix-turn-helix domain-containing protein	-
AMK02_RS28230	173575..173903	+	329	Protein_153	SRPBCC domain-containing protein	-
AMK02_RS28235	174006..174371	+	366	WP_064808827.1	hypothetical protein	-
AMK02_RS28240	174416..175249	+	834	WP_064808262.1	aminoglycoside phosphotransferase family protein	-
AMK02_RS28245	175400..175678	+	279	WP_064808264.1	DUF1778 domain-containing protein	Antitoxin
AMK02_RS28250	175684..176166	+	483	WP_064808266.1	GNAT family N-acetyltransferase	Toxin
AMK02_RS31580	176244..176654	-	411	WP_145924258.1	hypothetical protein	-
AMK02_RS28260	176850..177734	+	885	WP_064808268.1	NmrA family NAD(P)-binding protein	-
AMK02_RS28265	177784..178554	-	771	WP_064808270.1	glucose 1-dehydrogenase	-
AMK02_RS28270	178752..180686	+	1935	WP_064808272.1	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..506687	506687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-80)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17104.67 Da
• Isoelectric Point: 7.3826

>T59013 WP_064808266.1 NZ_CP013606:175684-176166 [Rhizobium sp. N731]
MLSPPALLGEAHDLQLFDSGHDGLDEWLRRRARANQVSGASRTYVVCEGERVIGYYCLSSGALAVADAPGAVRRNMPDPV
PMAVLGRLAIDLNWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFYEHYGFVASPANPMTLVLSLKARQA

Nucleotide

• Length: 483 bp

>T59013 NZ_CP013606:175684-176166 [Rhizobium sp. N731]
ATGTTGTCACCTCCGGCGCTTCTCGGAGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGATGGTCTCGATGAATG
GCTGCGCCGCCGGGCGCGGGCCAATCAGGTCAGCGGCGCGTCGCGAACCTACGTGGTCTGCGAGGGCGAGCGTGTCATCG
GTTACTATTGCCTGTCATCGGGGGCGCTCGCGGTCGCGGACGCGCCGGGAGCTGTCCGGCGCAATATGCCCGACCCCGTT
CCTATGGCAGTGCTCGGCCGTCTTGCTATCGATCTGAACTGGCAGGGAAAAGGCATTGGAGCTGCGCTGTTGCAAGACGC
CGTTTTGCGCACCGGACAGGCGGCCCATATCATGGGCATTCGTGGCCTTCTGGTTCACGCAATCTCCGACGAAGCCAAGG
CCTTTTACGAGCATTACGGGTTCGTCGCGTCGCCGGCAAACCCTATGACCTTGGTGTTATCGTTGAAAGCACGGCAGGCT
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10269.54 Da
• Isoelectric Point: 6.4818

>AT59013 WP_064808264.1 NZ_CP013606:175400-175678 [Rhizobium sp. N731]
MASKNATDTHKHAVNLKVRDDIRGLIDRAAKAHGKTRSDFMIDAARRAAEDALLDQTLVQVDPDSYEHYLAVLDQPPSGE
GFERLMNAPKPW

Nucleotide

• Length: 279 bp

>AT59013 NZ_CP013606:175400-175678 [Rhizobium sp. N731]
ATGGCCAGCAAAAACGCGACCGATACCCACAAACACGCCGTCAATTTGAAGGTTCGTGACGACATTCGCGGCCTGATCGA
CCGTGCAGCCAAGGCTCACGGGAAAACCCGCTCGGATTTCATGATCGACGCCGCGCGCCGCGCCGCCGAAGACGCGCTTC
TCGATCAGACATTGGTGCAAGTCGATCCCGATAGCTATGAACACTATCTGGCCGTCCTCGACCAGCCGCCCAGCGGGGAA
GGTTTTGAGCGGCTGATGAATGCTCCCAAACCCTGGTAG