Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 140344..140924 Replicon plasmid pRspN731e
Accession NZ_CP013606
Organism Rhizobium sp. N731

Toxin (Protein)


Gene name vapC Uniprot ID A0A192M693
Locus tag AMK02_RS28090 Protein ID WP_064808214.1
Coordinates 140344..140727 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0T6ZM79
Locus tag AMK02_RS28095 Protein ID WP_017958375.1
Coordinates 140724..140924 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK02_RS28075 135830..137029 + 1200 WP_064808209.1 mandelate racemase/muconate lactonizing enzyme family protein -
AMK02_RS28080 137092..137391 - 300 WP_064808211.1 hypothetical protein -
AMK02_RS28085 137623..139740 + 2118 WP_064808212.1 carboxy terminal-processing peptidase -
AMK02_RS28090 140344..140727 - 384 WP_064808214.1 type II toxin-antitoxin system VapC family toxin Toxin
AMK02_RS28095 140724..140924 - 201 WP_017958375.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
AMK02_RS30925 141195..141440 + 246 Protein_126 PAS domain S-box protein -
AMK02_RS28100 141732..142604 + 873 Protein_127 MurR/RpiR family transcriptional regulator -
AMK02_RS28105 142601..143317 - 717 WP_064808218.1 aspartate/glutamate racemase family protein -
AMK02_RS28110 143314..144231 - 918 WP_064808220.1 ABC transporter substrate-binding protein -
AMK02_RS28115 144241..145422 - 1182 WP_064808222.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..506687 506687


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14151.26 Da        Isoelectric Point: 6.8446

>T59012 WP_064808214.1 NZ_CP013606:c140727-140344 [Rhizobium sp. N731]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAIIGELALGSLRNRSSVIAFLAAQREALVATHDEVMMMIDRHAIFSM
GIGYTDAHLMASVLLDQRAALWTRDKRLRAAAEKAGASLHAPDNARN

Download         Length: 384 bp

>T59012 NZ_CP013606:c140727-140344 [Rhizobium sp. N731]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCTTATGCCACCCAGCCATAATCGGAGAGCTCGCACTTGGCAGCCTTCGAAATCGTAGTAGCGTCATAGCTTTCC
TGGCGGCTCAGCGCGAAGCGCTCGTTGCGACGCACGACGAAGTCATGATGATGATTGACCGCCACGCCATTTTCAGTATG
GGCATTGGCTACACAGATGCCCACTTGATGGCCTCAGTTCTCCTCGATCAACGAGCGGCCTTATGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGCGCTTCACTGCACGCCCCTGACAACGCGCGAAACTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7124.23 Da        Isoelectric Point: 11.3760

>AT59012 WP_017958375.1 NZ_CP013606:c140924-140724 [Rhizobium sp. N731]
MRSTINLDDTLMERARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT59012 NZ_CP013606:c140924-140724 [Rhizobium sp. N731]
ATGCGATCGACTATCAATCTCGACGACACCCTAATGGAAAGGGCCAGGTCCCTAACCGGCACGAAAGAAACCGCCGCTCT
GGTCCGCCAAGCTTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCATCGCTCTCGGCGGAACTATGCCGGATG
CGGAGGCAGCCCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M693

Antitoxin

Source ID Structure
AlphaFold DB A0A0T6ZM79

References