Detailed information of TA system TA059011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 274292..274827
• Replicon: plasmid pRspN731d
• Accession: NZ_CP013605
• Organism: Rhizobium sp. N731

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMK02_RS26530
• Protein ID: WP_064807623.1
• Coordinates: 274292..274567 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A192M8M4
• Locus tag: AMK02_RS26535
• Protein ID: WP_064807625.1
• Coordinates: 274567..274827 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK02_RS26510	269501..270427	-	927	WP_064807615.1	sugar ABC transporter permease	-
AMK02_RS26515	270480..271700	-	1221	WP_064807617.1	sugar ABC transporter substrate-binding protein	-
AMK02_RS26520	271750..273069	-	1320	WP_064807619.1	alpha-L-fucosidase	-
AMK02_RS26525	273278..274285	+	1008	WP_064807621.1	LacI family transcriptional regulator	-
AMK02_RS26530	274292..274567	-	276	WP_064807623.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMK02_RS26535	274567..274827	-	261	WP_064807625.1	ribbon-helix-helix domain-containing protein	Antitoxin
AMK02_RS26540	275127..275855	+	729	WP_064807627.1	FadR family transcriptional regulator	-
AMK02_RS26545	276108..277121	+	1014	WP_064807981.1	ABC transporter substrate-binding protein	-
AMK02_RS26550	277217..278035	+	819	WP_004677388.1	ABC transporter ATP-binding protein	-
AMK02_RS26555	278047..278928	+	882	WP_064807629.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..488789	488789

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10788.36 Da
• Isoelectric Point: 9.5543

>T59011 WP_064807623.1 NZ_CP013605:c274567-274292 [Rhizobium sp. N731]
MEIKWTSKAVSDIARLYDFLSPVDRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
ALRLWHTREDR

Nucleotide

• Length: 276 bp

>T59011 NZ_CP013605:c274567-274292 [Rhizobium sp. N731]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGGTCGACCGGCGAGC
TGCGGCCCGTACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCCACAATCTAT
GCATTGCGGCTTTGGCACACGCGCGAGGATCGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9670.88 Da
• Isoelectric Point: 4.6135

>AT59011 WP_064807625.1 NZ_CP013605:c274827-274567 [Rhizobium sp. N731]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLPR

Nucleotide

• Length: 261 bp

>AT59011 NZ_CP013605:c274827-274567 [Rhizobium sp. N731]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGATGGTGACGAG
CCGATTTCGTTGCCCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A192M8M4