Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/ParE-RHH
Location 290028..290584 Replicon plasmid pRspN731b
Accession NZ_CP013603
Organism Rhizobium sp. N731

Toxin (Protein)


Gene name parE Uniprot ID A0A192M462
Locus tag AMK02_RS23060 Protein ID WP_064807096.1
Coordinates 290028..290318 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A192M4C2
Locus tag AMK02_RS23065 Protein ID WP_064807097.1
Coordinates 290306..290584 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK02_RS23030 285109..285876 - 768 WP_064807087.1 LamB/YcsF family protein -
AMK02_RS23035 285898..286893 - 996 WP_064807089.1 biotin-dependent carboxyltransferase family protein -
AMK02_RS23040 286890..287597 - 708 WP_064807091.1 5-oxoprolinase subunit PxpB -
AMK02_RS23045 287594..288019 - 426 WP_064807198.1 hypothetical protein -
AMK02_RS23050 288016..289410 - 1395 WP_064807093.1 acetyl-CoA carboxylase biotin carboxylase subunit -
AMK02_RS23055 289410..289829 - 420 WP_064807094.1 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit -
AMK02_RS23060 290028..290318 - 291 WP_064807096.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMK02_RS23065 290306..290584 - 279 WP_064807097.1 CopG family ribbon-helix-helix protein Antitoxin
AMK02_RS23070 290722..291792 - 1071 WP_064807099.1 LacI family transcriptional regulator -
AMK02_RS23075 292012..292962 + 951 WP_064807101.1 sugar phosphate isomerase/epimerase -
AMK02_RS23080 293021..294253 + 1233 WP_064807102.1 ABC transporter substrate-binding protein -
AMK02_RS23085 294356..295243 + 888 WP_064807104.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..336117 336117


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-94)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11030.78 Da        Isoelectric Point: 9.6206

>T59010 WP_064807096.1 NZ_CP013603:c290318-290028 [Rhizobium sp. N731]
MPQVIFSPAAIRDLERLREFLRPKNPSVAKRAGETILKGVSVLGAHPHIGRLIEDMPEQYREWLIDFRDSGYVARYRMDG
DILTILAVRHQKEAGF

Download         Length: 291 bp

>T59010 NZ_CP013603:c290318-290028 [Rhizobium sp. N731]
ATGCCACAAGTGATCTTCTCCCCGGCCGCAATCCGCGATCTTGAGCGGCTTCGGGAATTCTTGCGACCGAAAAACCCGTC
AGTCGCCAAGCGGGCAGGCGAAACTATTCTCAAAGGCGTGAGCGTCCTCGGTGCGCATCCCCATATAGGACGCCTCATCG
AGGATATGCCCGAGCAATACCGGGAATGGCTCATCGATTTCAGAGATAGCGGGTACGTCGCTCGTTATCGTATGGATGGC
GACATTTTGACGATCCTCGCCGTGCGGCATCAGAAGGAAGCCGGGTTCTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10667.86 Da        Isoelectric Point: 4.7901

>AT59010 WP_064807097.1 NZ_CP013603:c290584-290306 [Rhizobium sp. N731]
MASPTSLKLDDELKGRVQQLAEARRRSSHWIMREAIAQYVEREEKREALRQETLEAWNEFEATGLHVTGAEVEKWLSTWG
TDDELSAPECHK

Download         Length: 279 bp

>AT59010 NZ_CP013603:c290584-290306 [Rhizobium sp. N731]
ATGGCATCGCCAACCTCGCTCAAGCTCGATGACGAGCTGAAGGGCCGCGTCCAACAACTGGCCGAGGCGCGCCGCCGATC
GTCCCACTGGATCATGCGTGAAGCCATTGCGCAATATGTCGAGCGCGAGGAGAAGCGCGAGGCGCTGCGGCAGGAGACGC
TTGAGGCCTGGAATGAATTTGAAGCGACGGGACTGCATGTCACCGGCGCCGAGGTCGAAAAATGGCTTTCGACATGGGGA
ACTGACGACGAGTTGTCCGCACCCGAATGCCACAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M462

Antitoxin

Source ID Structure
AlphaFold DB A0A192M4C2

References