Detailed information of TA system TA059008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 120258..120842
• Replicon: chromosome
• Accession: NZ_CP013601
• Organism: Rhizobium sp. N731

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AMK02_RS00570
• Protein ID: WP_064800213.1
• Coordinates: 120258..120440 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AMK02_RS00575
• Protein ID: WP_064800214.1
• Coordinates: 120447..120842 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK02_RS00540	115568..116041	-	474	WP_064800209.1	hypothetical protein	-
AMK02_RS00545	116219..116854	+	636	WP_064805816.1	trimeric intracellular cation channel family protein	-
AMK02_RS00550	116870..117289	-	420	WP_064800210.1	transcriptional repressor	-
AMK02_RS00555	117592..118107	+	516	WP_020920068.1	3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA	-
AMK02_RS00560	118153..119385	+	1233	WP_064800211.1	beta-ketoacyl-ACP synthase I	-
AMK02_RS00565	119390..120196	+	807	WP_064800212.1	enoyl-ACP reductase FabI	-
AMK02_RS00570	120258..120440	+	183	WP_064800213.1	type II toxin-antitoxin system HicA family toxin	Toxin
AMK02_RS00575	120447..120842	+	396	WP_064800214.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AMK02_RS00580	120846..121862	-	1017	WP_064800215.1	class I SAM-dependent methyltransferase	-
AMK02_RS00585	121938..124076	-	2139	WP_011423507.1	polyribonucleotide nucleotidyltransferase	-
AMK02_RS00590	124427..124696	-	270	WP_009997779.1	30S ribosomal protein S15	-
AMK02_RS00595	124846..125694	-	849	WP_064800216.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6738.86 Da
• Isoelectric Point: 11.9739

>T59008 WP_064800213.1 NZ_CP013601:120258-120440 [Rhizobium sp. N731]
IRTNQKIIARLKREGFEVVSIKGSHHKLRRGNQTVIVSHPRKDLPIGTALAIAKQAGWNE

Nucleotide

• Length: 183 bp

>T59008 NZ_CP013601:120258-120440 [Rhizobium sp. N731]
ATCCGGACGAACCAGAAGATTATCGCCAGGCTGAAGCGGGAGGGCTTCGAAGTGGTTTCAATCAAGGGCTCGCACCACAA
GCTTCGAAGAGGCAATCAGACCGTTATCGTTTCGCATCCCAGGAAGGATCTGCCGATCGGCACTGCGCTTGCGATTGCGA
AGCAGGCCGGCTGGAATGAGTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14320.11 Da
• Isoelectric Point: 4.5478

>AT59008 WP_064800214.1 NZ_CP013601:120447-120842 [Rhizobium sp. N731]
IKYFIALVHKDKNSAFGVSFPDLPSVFSAAGEEEDLTANAIDALRLWAEDETLPVPSSYDEISSRPDIREQLAAGAFLTR
VPFIEDTTRTVRANVTFDKGMLDAIDKAAKERGLTRSAFLASAARKEIEAA

Nucleotide

• Length: 396 bp

>AT59008 NZ_CP013601:120447-120842 [Rhizobium sp. N731]
ATAAAATATTTCATTGCGCTCGTACATAAGGACAAGAATAGCGCCTTCGGCGTCAGCTTTCCGGATCTGCCGTCGGTATT
CTCCGCGGCAGGTGAGGAAGAGGATCTTACCGCAAACGCCATCGACGCACTTCGTCTCTGGGCGGAGGATGAAACCTTGC
CGGTACCGTCCTCCTACGACGAGATCAGTTCGAGACCGGATATCCGCGAGCAGCTGGCTGCAGGCGCTTTTCTGACGCGG
GTTCCCTTTATCGAAGATACGACGCGCACCGTTAGAGCCAATGTCACTTTCGACAAAGGCATGCTTGACGCGATCGATAA
GGCCGCAAAGGAGCGCGGCCTTACCCGTTCGGCTTTCCTTGCAAGCGCTGCCCGCAAGGAGATCGAGGCCGCTTGA