Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 429771..430309 Replicon plasmid pRspN741e
Accession NZ_CP013600
Organism Rhizobium sp. N741

Toxin (Protein)


Gene name relE Uniprot ID A0A192M8I7
Locus tag AMK03_RS29760 Protein ID WP_064812708.1
Coordinates 429771..430049 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A192M8M4
Locus tag AMK03_RS29765 Protein ID WP_064807625.1
Coordinates 430049..430309 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK03_RS29740 424983..425909 - 927 WP_064812700.1 sugar ABC transporter permease -
AMK03_RS29745 425962..427182 - 1221 WP_064815955.1 sugar ABC transporter substrate-binding protein -
AMK03_RS29750 427232..428551 - 1320 WP_064812704.1 alpha-L-fucosidase -
AMK03_RS29755 428760..429767 + 1008 WP_064812706.1 LacI family transcriptional regulator -
AMK03_RS29760 429771..430049 - 279 WP_064812708.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMK03_RS29765 430049..430309 - 261 WP_064807625.1 ribbon-helix-helix domain-containing protein Antitoxin
AMK03_RS29770 430609..431337 + 729 WP_064807627.1 FadR family transcriptional regulator -
AMK03_RS29775 431590..432603 + 1014 WP_064813203.1 ABC transporter substrate-binding protein -
AMK03_RS29780 432699..433517 + 819 WP_004677388.1 ABC transporter ATP-binding protein -
AMK03_RS29785 433529..434410 + 882 WP_064812710.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..752910 752910


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10875.44 Da        Isoelectric Point: 9.5543

>T59007 WP_064812708.1 NZ_CP013600:c430049-429771 [Rhizobium sp. N741]
MEIKWTSKAVSDIARLYDFLSPVDRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
ALRLWHTREDRS

Download         Length: 279 bp

>T59007 NZ_CP013600:c430049-429771 [Rhizobium sp. N741]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGGTCGACCGGCGAGC
TGCGGCCCGTACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GCATTGCGGCTTTGGCATACGCGCGAGGATCGGTCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9670.88 Da        Isoelectric Point: 4.6135

>AT59007 WP_064807625.1 NZ_CP013600:c430309-430049 [Rhizobium sp. N741]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLPR

Download         Length: 261 bp

>AT59007 NZ_CP013600:c430309-430049 [Rhizobium sp. N741]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAACAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGATGGTGACGAG
CCGATTTCGTTGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M8I7

Antitoxin

Source ID Structure
AlphaFold DB A0A192M8M4

References