Detailed information of TA system TA059001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 119909..120465
• Replicon: chromosome
• Accession: NZ_CP013595
• Organism: Rhizobium sp. N741

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AMK03_RS00570
• Protein ID: WP_064809224.1
• Coordinates: 119909..120073 (+)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A192LQ69
• Locus tag: AMK03_RS00575
• Protein ID: WP_064809225.1
• Coordinates: 120070..120465 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK03_RS00540	115157..115630	-	474	WP_064800209.1	hypothetical protein	-
AMK03_RS00545	115808..116443	+	636	WP_064805816.1	trimeric intracellular cation channel family protein	-
AMK03_RS00550	116459..116878	-	420	WP_064800210.1	transcriptional repressor	-
AMK03_RS00555	117180..117695	+	516	WP_020920068.1	3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA	-
AMK03_RS00560	117741..118973	+	1233	WP_064809222.1	beta-ketoacyl-ACP synthase I	-
AMK03_RS00565	118978..119784	+	807	WP_064809223.1	enoyl-ACP reductase FabI	-
AMK03_RS00570	119909..120073	+	165	WP_064809224.1	type II toxin-antitoxin system HicA family toxin	Toxin
AMK03_RS00575	120070..120465	+	396	WP_064809225.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AMK03_RS00580	120469..121485	-	1017	WP_064809226.1	class I SAM-dependent methyltransferase	-
AMK03_RS00585	121616..123754	-	2139	WP_011423507.1	polyribonucleotide nucleotidyltransferase	-
AMK03_RS00590	124123..124392	-	270	WP_009997779.1	30S ribosomal protein S15	-
AMK03_RS00595	124542..125390	-	849	WP_064809227.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-48)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 5894.84 Da
• Isoelectric Point: 10.9062

>T59001 WP_064809224.1 NZ_CP013595:119909-120073 [Rhizobium sp. N741]
MKREGFEVVSIKGSHHKLRRGTETIIVPHPKKDLPTGTALAIAKQAGWSVSDKS

Nucleotide

• Length: 165 bp

>T59001 NZ_CP013595:119909-120073 [Rhizobium sp. N741]
TTGAAGCGCGAGGGCTTTGAAGTTGTTTCGATCAAAGGCTCGCACCACAAGCTTCGTAGAGGCACTGAGACCATCATCGT
TCCCCACCCGAAGAAGGATTTGCCGACCGGCACCGCGCTTGCCATTGCCAAGCAGGCCGGATGGAGTGTGAGTGACAAAT
CATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14551.40 Da
• Isoelectric Point: 4.5667

>AT59001 WP_064809225.1 NZ_CP013595:120070-120465 [Rhizobium sp. N741]
MRYFIAIVHKDRDSAFGISFPDLPAVFSAADVEEDLTAHAIEALRLWAEDETLPMPSSYDEISSRQDIREQLTAGAFLTR
VPFIEDTTRTVRANVTFDKGMLDAIDKAAKERGLTRSAFLASAARKEIEAA

Nucleotide

• Length: 396 bp

>AT59001 NZ_CP013595:120070-120465 [Rhizobium sp. N741]
ATGAGATATTTCATCGCCATCGTGCATAAGGACAGAGACAGCGCTTTTGGTATCAGCTTTCCAGATCTGCCGGCGGTATT
CTCCGCAGCCGATGTGGAAGAAGACCTGACTGCGCATGCCATCGAAGCACTTCGCCTCTGGGCGGAGGATGAAACTCTGC
CGATGCCCTCCTCATACGACGAGATCAGCTCTCGGCAAGATATCCGCGAGCAACTGACCGCGGGGGCCTTTCTGACACGT
GTTCCCTTTATCGAAGACACGACGCGCACAGTTCGAGCCAATGTCACCTTCGACAAAGGCATGCTTGATGCGATCGACAA
GGCCGCGAAAGAACGTGGCTTGACCCGCTCGGCTTTCCTTGCAAGTGCGGCCCGCAAGGAAATCGAAGCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A192LQ69