Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/ParE-RHH
Location 269159..269715 Replicon plasmid pRspN871a
Accession NZ_CP013591
Organism Rhizobium sp. N871

Toxin (Protein)


Gene name parE Uniprot ID A0A192M462
Locus tag AMK04_RS22860 Protein ID WP_064807096.1
Coordinates 269159..269449 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A192M4C2
Locus tag AMK04_RS22865 Protein ID WP_064807097.1
Coordinates 269437..269715 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK04_RS22830 264240..265007 - 768 WP_064807087.1 LamB/YcsF family protein -
AMK04_RS22835 265029..266024 - 996 WP_064807089.1 biotin-dependent carboxyltransferase family protein -
AMK04_RS22840 266021..266728 - 708 WP_064807091.1 5-oxoprolinase subunit PxpB -
AMK04_RS22845 266725..267150 - 426 WP_064807198.1 hypothetical protein -
AMK04_RS22850 267147..268541 - 1395 WP_064807093.1 acetyl-CoA carboxylase biotin carboxylase subunit -
AMK04_RS22855 268541..268960 - 420 WP_064807094.1 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit -
AMK04_RS22860 269159..269449 - 291 WP_064807096.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMK04_RS22865 269437..269715 - 279 WP_064807097.1 CopG family ribbon-helix-helix protein Antitoxin
AMK04_RS22870 269838..270908 - 1071 WP_064811910.1 LacI family transcriptional regulator -
AMK04_RS22875 271129..272079 + 951 WP_064811911.1 sugar phosphate isomerase/epimerase -
AMK04_RS22880 272138..273370 + 1233 WP_064811912.1 ABC transporter substrate-binding protein -
AMK04_RS22885 273473..274360 + 888 WP_064811913.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..315154 315154


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-94)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11030.78 Da        Isoelectric Point: 9.6206

>T58996 WP_064807096.1 NZ_CP013591:c269449-269159 [Rhizobium sp. N871]
MPQVIFSPAAIRDLERLREFLRPKNPSVAKRAGETILKGVSVLGAHPHIGRLIEDMPEQYREWLIDFRDSGYVARYRMDG
DILTILAVRHQKEAGF

Download         Length: 291 bp

>T58996 NZ_CP013591:c269449-269159 [Rhizobium sp. N871]
ATGCCACAAGTGATCTTCTCCCCGGCCGCAATCCGCGATCTTGAGCGGCTTCGGGAATTCTTGCGACCGAAAAACCCGTC
AGTCGCCAAGCGGGCAGGCGAAACTATTCTCAAAGGCGTGAGCGTCCTCGGTGCGCATCCCCATATAGGACGCCTCATCG
AGGATATGCCCGAGCAATACCGGGAATGGCTCATCGATTTCAGAGATAGCGGGTACGTCGCTCGTTATCGTATGGATGGC
GACATTTTGACGATCCTCGCCGTGCGGCATCAGAAGGAAGCCGGGTTCTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10667.86 Da        Isoelectric Point: 4.7901

>AT58996 WP_064807097.1 NZ_CP013591:c269715-269437 [Rhizobium sp. N871]
MASPTSLKLDDELKGRVQQLAEARRRSSHWIMREAIAQYVEREEKREALRQETLEAWNEFEATGLHVTGAEVEKWLSTWG
TDDELSAPECHK

Download         Length: 279 bp

>AT58996 NZ_CP013591:c269715-269437 [Rhizobium sp. N871]
ATGGCATCGCCAACCTCGCTCAAGCTCGATGACGAGCTGAAGGGCCGCGTCCAACAACTGGCCGAGGCGCGCCGCCGATC
GTCCCACTGGATCATGCGTGAAGCCATTGCGCAATATGTCGAGCGCGAGGAGAAGCGCGAGGCGCTGCGGCAGGAGACGC
TTGAGGCCTGGAATGAATTTGAAGCGACGGGACTGCATGTCACCGGCGCCGAGGTCGAAAAATGGCTTTCGACCTGGGGA
ACTGACGACGAGTTGTCCGCACCCGAATGCCACAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M462

Antitoxin

Source ID Structure
AlphaFold DB A0A192M4C2

References