Detailed information of TA system TA058991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 550554..551089
• Replicon: plasmid pRphaN161d
• Accession: NZ_CP013589
• Organism: Rhizobium phaseoli strain N161

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC78_RS29685
• Protein ID: WP_064837557.1
• Coordinates: 550554..550829 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2AEZ7
• Locus tag: AMC78_RS29690
• Protein ID: WP_004677393.1
• Coordinates: 550829..551089 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC78_RS29665	545745..546671	-	927	WP_012490002.1	sugar ABC transporter permease	-
AMC78_RS29670	546739..547959	-	1221	WP_004677398.1	sugar ABC transporter substrate-binding protein	-
AMC78_RS29675	548009..549331	-	1323	WP_012490003.1	alpha-L-fucosidase	-
AMC78_RS29680	549540..550547	+	1008	WP_004677397.1	LacI family transcriptional regulator	-
AMC78_RS29685	550554..550829	-	276	WP_064837557.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMC78_RS29690	550829..551089	-	261	WP_004677393.1	ribbon-helix-helix domain-containing protein	Antitoxin
AMC78_RS29695	551389..552117	+	729	WP_004677391.1	FadR family transcriptional regulator	-
AMC78_RS29700	552408..553421	+	1014	WP_016735851.1	ABC transporter substrate-binding protein	-
AMC78_RS29705	553517..554335	+	819	WP_004677388.1	ABC transporter ATP-binding protein	-
AMC78_RS29710	554347..555228	+	882	WP_012490008.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gmd	1..1048055	1048055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10858.49 Da
• Isoelectric Point: 9.5543

>T58991 WP_064837557.1 NZ_CP013589:c550829-550554 [Rhizobium phaseoli]
MEIKWTSKVVSDIARLYDFLSPIDRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T58991 NZ_CP013589:c550829-550554 [Rhizobium phaseoli]
ATGGAAATCAAGTGGACGAGCAAAGTTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGATCGACCGGCGAGC
TGCGGCCCGAACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GTATTGCGGCTTTGGCATACGCGCGAGGATCGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9686.93 Da
• Isoelectric Point: 4.6135

>AT58991 WP_004677393.1 NZ_CP013589:c551089-550829 [Rhizobium phaseoli]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLLR

Nucleotide

• Length: 261 bp

>AT58991 NZ_CP013589:c551089-550829 [Rhizobium phaseoli]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGACGGTGACGAG
CCGATTTCGTTGCTCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U3CQR5