Detailed information of TA system TA058989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 122889..123469
• Replicon: plasmid pRphaN161c
• Accession: NZ_CP013588
• Organism: Rhizobium phaseoli strain N161

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AMC78_RS25815
• Protein ID: WP_064836873.1
• Coordinates: 122889..123272 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AMC78_RS25820
• Protein ID: WP_064836874.1
• Coordinates: 123269..123469 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC78_RS32940	119660..120532	-	873	WP_081279286.1	SIR2 family protein	-
AMC78_RS25810	120848..122809	+	1962	WP_064837139.1	elongation factor G	-
AMC78_RS25815	122889..123272	-	384	WP_064836873.1	type II toxin-antitoxin system VapC family toxin	Toxin
AMC78_RS25820	123269..123469	-	201	WP_064836874.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AMC78_RS25825	123805..125922	+	2118	WP_064836876.1	carboxy terminal-processing peptidase	-
AMC78_RS25830	126080..126325	+	246	WP_064819986.1	DUF1778 domain-containing protein	-
AMC78_RS25835	126322..126816	+	495	WP_064836877.1	GNAT family N-acetyltransferase	-
AMC78_RS25840	126946..127842	+	897	WP_064836878.1	GGDEF domain-containing protein	-
AMC78_RS25845	127855..128220	-	366	WP_064836879.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..407297	407297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14266.34 Da
• Isoelectric Point: 6.8445

>T58989 WP_064836873.1 NZ_CP013588:c123272-122889 [Rhizobium phaseoli]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALSSLRDRSNVIAFLAPQRQAFVATHDEVMMMIDRHAIFSM
GIGYTDAHLIASVLLDQRAALWTRDKRLRAAAEKAGASLHTPDNARN

Nucleotide

• Length: 384 bp

>T58989 NZ_CP013588:c123272-122889 [Rhizobium phaseoli]
GTGATACTTGCCGACACTTCCATCTGGATTGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCTTATGCCATCCGGCCGTGATCGGAGAACTCGCACTTAGCAGCCTTCGGGATCGCAGTAACGTCATAGCTTTCC
TGGCGCCCCAGCGCCAAGCGTTCGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGCCACGCCATTTTCAGCATG
GGCATTGGCTACACAGATGCCCATTTGATCGCTTCGGTTCTACTCGATCAGCGAGCCGCCTTATGGACGAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTTCACACGCCCGACAATGCGCGAAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7214.35 Da
• Isoelectric Point: 11.3760

>AT58989 WP_064836874.1 NZ_CP013588:c123469-123269 [Rhizobium phaseoli]
MRSTINLDDTLMERARFLTGTKETATLVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT58989 NZ_CP013588:c123469-123269 [Rhizobium phaseoli]
ATGCGATCAACTATCAATCTCGACGACACCCTCATGGAAAGGGCCAGATTTCTAACCGGCACGAAAGAAACCGCGACTCT
GGTCCGCCAAGCGTTGGAAACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCATCGCGCTAGGCGGAACTATGCCGGATG
CGGAGGCAGCTCCACGCCGCCGGAGCGCAGCCGCCAAGTGA