Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 873093..873811 Replicon chromosome
Accession NZ_CP013585
Organism Rhizobium phaseoli strain N161

Toxin (Protein)


Gene name vapC Uniprot ID B3PRC0
Locus tag AMC78_RS04265 Protein ID WP_012482827.1
Coordinates 873093..873551 (-) Length 153 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID B3PRC1
Locus tag AMC78_RS04270 Protein ID WP_012482828.1
Coordinates 873548..873811 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC78_RS04245 868881..870518 - 1638 WP_012482824.1 chaperonin GroEL -
AMC78_RS04250 870589..870885 - 297 WP_004675403.1 co-chaperone GroES -
AMC78_RS04255 871220..872068 + 849 WP_012482825.1 TIGR01459 family HAD-type hydrolase -
AMC78_RS04260 872083..873066 + 984 WP_064836247.1 bifunctional riboflavin kinase/FAD synthetase -
AMC78_RS04265 873093..873551 - 459 WP_012482827.1 type II toxin-antitoxin system VapC family toxin Toxin
AMC78_RS04270 873548..873811 - 264 WP_012482828.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AMC78_RS04275 874163..877069 + 2907 WP_064836249.1 isoleucine--tRNA ligase -
AMC78_RS04280 877201..877830 + 630 WP_012482830.1 hypothetical protein -
AMC78_RS04285 878102..878539 - 438 WP_064833944.1 nucleoside deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-127)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(7-58)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17015.19 Da        Isoelectric Point: 6.8817

>T58986 WP_012482827.1 NZ_CP013585:c873551-873093 [Rhizobium phaseoli]
VIGWLLDTNVIAALINPNGAPSVKSWAAAQDENQMFISVLTLAEYDKGIENLPEDDRNRYRYIAARDALEERFSQRTLSL
SDAAVRHWGAISGRIKLRTGHAPPVIDTMLAATAIEHNLYLVTRNAKDTRFSGAAVFDPWTHDPNQFPLSRR

Download         Length: 459 bp

>T58986 NZ_CP013585:c873551-873093 [Rhizobium phaseoli]
GTGATCGGATGGCTGCTCGACACCAACGTCATTGCGGCGCTGATCAATCCGAACGGCGCGCCCTCTGTGAAGTCTTGGGC
TGCCGCGCAGGACGAGAACCAGATGTTCATCAGCGTCCTCACCCTCGCAGAATATGACAAAGGCATCGAAAATCTGCCTG
AAGACGACCGAAACCGCTATCGTTATATAGCGGCGCGTGACGCTCTGGAGGAGCGGTTCTCACAGCGTACTCTCTCGCTG
AGCGACGCGGCTGTCAGGCATTGGGGCGCTATTTCAGGGCGCATAAAATTGCGAACCGGACATGCGCCACCGGTCATCGA
CACAATGTTGGCCGCCACCGCCATTGAGCACAATCTCTATCTGGTGACGCGGAACGCAAAGGATACGCGCTTCTCCGGCG
CGGCAGTTTTCGATCCGTGGACACACGATCCGAATCAATTTCCATTGAGCCGGAGATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10152.71 Da        Isoelectric Point: 8.5763

>AT58986 WP_012482828.1 NZ_CP013585:c873811-873548 [Rhizobium phaseoli]
MRKIVKQSWKLEDAKARFSEVVRRARSEGPQRVTVRGRDSVVIISVEELERLTKAEPKKPLVDFMESLALKELDLEREPD
YGRDVEL

Download         Length: 264 bp

>AT58986 NZ_CP013585:c873811-873548 [Rhizobium phaseoli]
ATGAGAAAAATTGTAAAGCAAAGCTGGAAGCTCGAGGACGCCAAAGCGCGCTTCAGCGAAGTAGTACGCCGTGCCCGCAG
CGAAGGGCCGCAACGCGTGACAGTGCGCGGCAGAGACAGCGTCGTCATCATCAGCGTCGAAGAACTCGAACGGTTGACGA
AGGCCGAGCCCAAAAAGCCTTTGGTCGATTTCATGGAGAGCCTCGCTCTGAAAGAGCTCGATCTCGAGCGGGAGCCCGAT
TACGGGCGGGATGTCGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M3GMR1

Antitoxin

Source ID Structure
AlphaFold DB A0A0M3GPB5

References