Detailed information of TA system TA058985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 836373..836971
• Replicon: plasmid pRphaN261d
• Accession: NZ_CP013584
• Organism: Rhizobium phaseoli strain N261

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC79_RS33180
• Protein ID: WP_081279432.1
• Coordinates: 836373..836666 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7X6J1Z1
• Locus tag: AMC79_RS30785
• Protein ID: WP_064820408.1
• Coordinates: 836663..836971 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC79_RS30765	831689..832450	-	762	WP_064820405.1	SDR family oxidoreductase	-
AMC79_RS30770	832590..833453	+	864	WP_064820406.1	LysR family transcriptional regulator	-
AMC79_RS30775	833464..833883	-	420	Protein_774	error-prone DNA polymerase	-
AMC79_RS34255	835186..835488	-	303	WP_185185227.1	diguanylate cyclase	-
AMC79_RS34260	835485..835859	-	375	WP_185185226.1	hypothetical protein	-
AMC79_RS33180	836373..836666	-	294	WP_081279432.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
AMC79_RS30785	836663..836971	-	309	WP_064820408.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AMC79_RS30790	837287..838075	-	789	WP_081279433.1	NAD(P)-dependent alcohol dehydrogenase	-
AMC79_RS30795	838260..838643	-	384	WP_064820409.1	DoxX family protein	-
AMC79_RS30800	838658..839506	-	849	WP_064820410.1	SDR family oxidoreductase	-
AMC79_RS30805	839642..840043	+	402	WP_064820580.1	helix-turn-helix transcriptional regulator	-
AMC79_RS30810	840084..840531	-	448	Protein_783	transposase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gmd	1..1074794	1074794
	-	flank	IS/Tn	-	-	840188..840400	212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10624.19 Da
• Isoelectric Point: 5.7996

>T58985 WP_081279432.1 NZ_CP013584:c836666-836373 [Rhizobium phaseoli]
VKLTWSVFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLIDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDTLPTS

Nucleotide

• Length: 294 bp

>T58985 NZ_CP013584:c836666-836373 [Rhizobium phaseoli]
GTGAAGCTTACCTGGTCTGTCTTCGCATTATCTGACCGCGACGCCATATTTACGTACATAGAAGCGGAAAATCCGTCAGC
CGCTATTCTGGTCGACGAACGGATCGTTGCTGCCGTTCGTCGGTTGATAGATTTTCCCGCAAGCGGTCGTGTTGGCAGAA
TTGCTGGAACCCGCGAACTGGTAATTAACGGTACGCCGTACGTCGCGGCTTATGCAATTACCGAAACAGCAGTCCGTATT
CTTCGCGTCCTCCATGGCGCGCAGGAATGGCCAGACACTTTGCCAACGAGCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11013.38 Da
• Isoelectric Point: 6.4721

>AT58985 WP_064820408.1 NZ_CP013584:c836971-836663 [Rhizobium phaseoli]
MAANALVQTRIDAEVRDRASAVLENMGLTVSDAVRILLTRTANEGALPLELLSGSEAHDAWFRTKVLEALNDTRPDVSDN
EAEAHFAARRAAARLKAGSRKS

Nucleotide

• Length: 309 bp

>AT58985 NZ_CP013584:c836971-836663 [Rhizobium phaseoli]
ATGGCTGCAAATGCACTCGTGCAAACTCGTATTGATGCCGAAGTCCGGGATCGGGCCTCCGCAGTGCTTGAAAACATGGG
GCTTACAGTATCGGACGCGGTTCGTATCTTGCTCACCCGGACGGCCAATGAGGGTGCGCTGCCACTCGAGCTTCTCTCAG
GCAGCGAGGCGCATGACGCGTGGTTTCGCACCAAGGTGCTTGAAGCGCTGAACGATACTCGACCAGACGTCTCGGATAAC
GAAGCCGAGGCACATTTTGCCGCACGTCGAGCGGCAGCCCGACTTAAAGCGGGTTCTCGAAAATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X6J1Z1