Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 577655..578190 Replicon plasmid pRphaN261d
Accession NZ_CP013584
Organism Rhizobium phaseoli strain N261

Toxin (Protein)


Gene name relE Uniprot ID A0A0M3GDG7
Locus tag AMC79_RS29610 Protein ID WP_016735850.1
Coordinates 577655..577930 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID F2AEZ7
Locus tag AMC79_RS29615 Protein ID WP_004677393.1
Coordinates 577930..578190 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC79_RS29590 572850..573776 - 927 WP_064820571.1 sugar ABC transporter permease -
AMC79_RS29595 573844..575060 - 1217 Protein_535 sugar ABC transporter substrate-binding protein -
AMC79_RS29600 575110..576432 - 1323 WP_012490003.1 alpha-L-fucosidase -
AMC79_RS29605 576641..577648 + 1008 WP_004677397.1 LacI family transcriptional regulator -
AMC79_RS29610 577655..577930 - 276 WP_016735850.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMC79_RS29615 577930..578190 - 261 WP_004677393.1 ribbon-helix-helix domain-containing protein Antitoxin
AMC79_RS29620 578490..579218 + 729 WP_064820310.1 FadR family transcriptional regulator -
AMC79_RS29625 579509..580522 + 1014 WP_016735851.1 ABC transporter substrate-binding protein -
AMC79_RS29630 580618..581436 + 819 WP_064820311.1 ABC transporter ATP-binding protein -
AMC79_RS29635 581448..582329 + 882 WP_016735853.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gmd 1..1074794 1074794


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10816.41 Da        Isoelectric Point: 9.5543

>T58983 WP_016735850.1 NZ_CP013584:c577930-577655 [Rhizobium phaseoli]
MEIKWTSKAVSDIARLYDFLSPVDRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
VLRLWHTREDR

Download         Length: 276 bp

>T58983 NZ_CP013584:c577930-577655 [Rhizobium phaseoli]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGGTCGACCGGCGAGC
TGCGGCCCGAACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGTCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GTATTGCGGCTTTGGCATACGCGCGAGGATCGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9686.93 Da        Isoelectric Point: 4.6135

>AT58983 WP_004677393.1 NZ_CP013584:c578190-577930 [Rhizobium phaseoli]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLLR

Download         Length: 261 bp

>AT58983 NZ_CP013584:c578190-577930 [Rhizobium phaseoli]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGACGGTGACGAG
CCGATTTCGTTGCTCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M3GDG7

Antitoxin

Source ID Structure
AlphaFold DB A0A2U3CQR5

References