Detailed information of TA system TA058973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 547732..548267
• Replicon: plasmid pRphaN671e
• Accession: NZ_CP013579
• Organism: Rhizobium phaseoli strain N671

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC80_RS30055
• Protein ID: WP_037145718.1
• Coordinates: 547732..548007 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2AEZ7
• Locus tag: AMC80_RS30060
• Protein ID: WP_004677393.1
• Coordinates: 548007..548267 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC80_RS30035	542923..543849	-	927	WP_029531794.1	sugar ABC transporter permease	-
AMC80_RS30040	543917..545137	-	1221	WP_037145720.1	sugar ABC transporter substrate-binding protein	-
AMC80_RS30045	545187..546509	-	1323	WP_012490003.1	alpha-L-fucosidase	-
AMC80_RS30050	546718..547725	+	1008	WP_010004849.1	LacI family transcriptional regulator	-
AMC80_RS30055	547732..548007	-	276	WP_037145718.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMC80_RS30060	548007..548267	-	261	WP_004677393.1	ribbon-helix-helix domain-containing protein	Antitoxin
AMC80_RS30065	548567..549295	+	729	WP_004677391.1	FadR family transcriptional regulator	-
AMC80_RS30070	549586..550599	+	1014	WP_012490006.1	ABC transporter substrate-binding protein	-
AMC80_RS30075	550695..551513	+	819	WP_037145716.1	ABC transporter ATP-binding protein	-
AMC80_RS30080	551525..552406	+	882	WP_004677386.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gmd	1..1189373	1189373

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10802.39 Da
• Isoelectric Point: 9.5543

>T58973 WP_037145718.1 NZ_CP013579:c548007-547732 [Rhizobium phaseoli]
MEIKWTSKAVSDIARLYDFLSPVDRRAAARTVQALAAAPARLIEQPRLGERLDEFDPQEVRRILVGRYEMRYEIQQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T58973 NZ_CP013579:c548007-547732 [Rhizobium phaseoli]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGGTCGACCGGCGAGC
TGCGGCCCGAACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGATG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GTATTGCGGCTTTGGCATACGCGCGAGGATCGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9686.93 Da
• Isoelectric Point: 4.6135

>AT58973 WP_004677393.1 NZ_CP013579:c548267-548007 [Rhizobium phaseoli]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLLR

Nucleotide

• Length: 261 bp

>AT58973 NZ_CP013579:c548267-548007 [Rhizobium phaseoli]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGACGGTGACGAG
CCGATTTCGTTGCTCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U3CQR5