Detailed information of TA system TA058949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 800286..800884
• Replicon: plasmid pRphaN841e
• Accession: NZ_CP013562
• Organism: Rhizobium phaseoli strain N841

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC83_RS33990
• Protein ID: WP_080574712.1
• Coordinates: 800286..800579 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2A2S3
• Locus tag: AMC83_RS30880
• Protein ID: WP_004669386.1
• Coordinates: 800576..800884 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC83_RS30865	795771..798299	+	2529	WP_064832388.1	methyl-accepting chemotaxis protein	-
AMC83_RS30870	798945..800126	-	1182	WP_037146451.1	Fic family protein	-
AMC83_RS33990	800286..800579	-	294	WP_080574712.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
AMC83_RS30880	800576..800884	-	309	WP_004669386.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AMC83_RS30885	801466..802455	+	990	WP_037146453.1	TIM barrel protein	-
AMC83_RS30890	802484..803524	+	1041	WP_037146455.1	substrate-binding domain-containing protein	-
AMC83_RS30895	803588..805099	+	1512	WP_004669413.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gmd	1..1201422	1201422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10596.14 Da
• Isoelectric Point: 5.7996

>T58949 WP_080574712.1 NZ_CP013562:c800579-800286 [Rhizobium phaseoli]
VKLTWSAFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLIDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDTLPTS

Nucleotide

• Length: 294 bp

>T58949 NZ_CP013562:c800579-800286 [Rhizobium phaseoli]
GTGAAGCTTACCTGGTCTGCCTTCGCATTATCGGATCGCGACGCCATATTCACATACATAGAAGCGGAAAATCCGTCAGC
CGCTATTCTGGTCGACGAACGGATCGTTGCTGCCGTTCGTCGGTTGATAGATTTTCCCGCGAGCGGTCGTGTCGGCAGAA
TTGCTGGGACCCGCGAACTGGTAATTAACGGTACGCCGTACGTCGCGGCGTATGCAATTACCGAAACAGCGGTCCGCATT
CTTCGCGTCCTCCATGGCGCGCAGGAATGGCCAGACACTTTGCCAACGAGCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11110.45 Da
• Isoelectric Point: 5.1751

>AT58949 WP_004669386.1 NZ_CP013562:c800884-800576 [Rhizobium phaseoli]
MAANALVQTRIDAEVRDRASAVLENMGLTVSDAVRILLTRTANEGALPLELFSGSEAHDAWFRTKVLEALNDTRPDVSDD
EVEAQFAKRRAAARLNAGSRKS

Nucleotide

• Length: 309 bp

>AT58949 NZ_CP013562:c800884-800576 [Rhizobium phaseoli]
ATGGCTGCAAATGCACTCGTGCAAACTCGTATTGATGCCGAAGTTCGGGATCGGGCCTCCGCAGTGCTTGAAAACATGGG
GCTTACAGTGTCGGACGCGGTTCGTATCTTGCTCACCCGGACGGCCAACGAGGGTGCGCTGCCACTCGAGCTTTTTTCAG
GCAGCGAGGCCCATGACGCTTGGTTTCGCACCAAGGTGCTTGAAGCGCTGAACGATACTCGACCGGACGTTTCGGATGAT
GAAGTCGAGGCACAATTTGCTAAACGTCGAGCGGCAGCCCGACTTAACGCGGGTTCTCGAAAATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U3CRA1