Detailed information of TA system TA058947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 133248..133984
• Replicon: plasmid pRphaN841c
• Accession: NZ_CP013560
• Organism: Rhizobium phaseoli strain N841

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: AMC83_RS23895
• Protein ID: WP_064832229.1
• Coordinates: 133490..133984 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: AMC83_RS23890
• Protein ID: WP_064832228.1
• Coordinates: 133248..133493 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC83_RS23875	130061..130444	-	384	WP_064832109.1	type II toxin-antitoxin system VapC family toxin	-
AMC83_RS23880	130441..130641	-	201	WP_064832110.1	type II toxin-antitoxin system VapB family antitoxin	-
AMC83_RS23885	130978..133095	+	2118	WP_064832111.1	carboxy terminal-processing peptidase	-
AMC83_RS23890	133248..133493	+	246	WP_064832228.1	DUF1778 domain-containing protein	Antitoxin
AMC83_RS23895	133490..133984	+	495	WP_064832229.1	GNAT family N-acetyltransferase	Toxin
AMC83_RS23900	134114..135010	+	897	WP_064832112.1	GGDEF domain-containing protein	-
AMC83_RS23905	135040..135384	-	345	WP_064832113.1	hypothetical protein	-
AMC83_RS23910	135411..135887	-	477	WP_064832114.1	hypothetical protein	-
AMC83_RS23915	135907..137127	-	1221	WP_064832115.1	hypothetical protein	-
AMC83_RS23920	137170..137937	-	768	WP_064832116.1	TfuA-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..407882	407882

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-73)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17755.38 Da
• Isoelectric Point: 9.0589

>T58947 WP_064832229.1 NZ_CP013560:133490-133984 [Rhizobium phaseoli]
VTLSRPAPLADHHELAEFSSGVAELDEWLRRRARANQASGASRTFVVCEGNRVIAYYALASGAVRQPEAPGRFRRNMPDP
IPVAVLGRLAIDQSHQGRGIGRALVRDAGLRLVNAAEILGIRGLLVHAISDDARAFYEAVGFLPLPSDPMMLMVGLHDLN
SALR

Nucleotide

• Length: 495 bp

>T58947 NZ_CP013560:133490-133984 [Rhizobium phaseoli]
GTGACGCTCAGCCGGCCGGCGCCTTTGGCCGATCATCACGAGCTGGCCGAATTCAGTTCCGGCGTGGCTGAACTGGACGA
ATGGCTGCGCCGCCGCGCCCGCGCCAATCAGGCGAGCGGCGCGTCACGCACCTTCGTCGTGTGCGAGGGAAACCGGGTCA
TCGCCTATTATGCGCTCGCCTCTGGAGCGGTGCGGCAGCCGGAAGCGCCCGGCCGTTTTCGGCGGAACATGCCCGACCCT
ATCCCGGTTGCCGTGCTTGGCCGCCTCGCCATCGATCAATCCCACCAGGGGCGCGGTATCGGCAGGGCATTGGTGCGCGA
TGCGGGCCTGCGCCTGGTCAACGCCGCAGAAATCCTCGGAATTCGCGGTCTGCTGGTGCATGCGATTTCCGACGACGCGC
GCGCCTTCTATGAGGCGGTCGGCTTCCTGCCGTTGCCATCCGATCCCATGATGCTGATGGTCGGGTTGCATGATCTCAAC
AGCGCGCTGCGGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9336.67 Da
• Isoelectric Point: 5.1972

>AT58947 WP_064832228.1 NZ_CP013560:133248-133493 [Rhizobium phaseoli]
MRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTVFTVSPEIYAEYLARLDAPAEPNERLKRTMSTKAPWDE
A

Nucleotide

• Length: 246 bp

>AT58947 NZ_CP013560:133248-133493 [Rhizobium phaseoli]
ATGCGCATCAAGCCTGCGACCCGGAATTTGATCGACCGTGCCGCCGAACTGCTTGGCAAGACGCGCACGGATTTCATGCT
GGAGGCTTCGGAGCGCCGGGCTGAGGAAGTGCTGCTCGACCGGACGGTATTCACGGTCAGCCCCGAGATCTATGCCGAAT
ATCTTGCCCGCCTGGATGCGCCGGCTGAGCCCAATGAGCGTCTGAAACGGACGATGTCGACCAAAGCGCCATGGGACGAA
GCGTGA