Detailed information of TA system TA058941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 824995..825593
• Replicon: plasmid pRphaN931d
• Accession: NZ_CP013556
• Organism: Rhizobium phaseoli strain N931

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC84_RS33185
• Protein ID: WP_080574712.1
• Coordinates: 824995..825288 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2A2S3
• Locus tag: AMC84_RS30370
• Protein ID: WP_004669386.1
• Coordinates: 825285..825593 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC84_RS30355	820480..823008	+	2529	WP_064824090.1	methyl-accepting chemotaxis protein	-
AMC84_RS30360	823654..824835	-	1182	WP_037146451.1	Fic family protein	-
AMC84_RS33185	824995..825288	-	294	WP_080574712.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
AMC84_RS30370	825285..825593	-	309	WP_004669386.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AMC84_RS30375	826175..827164	+	990	WP_037146453.1	TIM barrel protein	-
AMC84_RS30380	827193..828233	+	1041	WP_037146455.1	substrate-binding domain-containing protein	-
AMC84_RS30385	828297..829808	+	1512	WP_004669413.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..1165895	1165895

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10596.14 Da
• Isoelectric Point: 5.7996

>T58941 WP_080574712.1 NZ_CP013556:c825288-824995 [Rhizobium phaseoli]
VKLTWSAFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLIDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDTLPTS

Nucleotide

• Length: 294 bp

>T58941 NZ_CP013556:c825288-824995 [Rhizobium phaseoli]
GTGAAGCTTACCTGGTCTGCCTTCGCATTATCGGATCGCGACGCCATATTCACATACATAGAAGCGGAAAATCCGTCAGC
CGCTATTCTGGTCGACGAACGGATCGTTGCTGCCGTTCGTCGGTTGATAGATTTTCCCGCGAGCGGTCGTGTCGGCAGAA
TTGCTGGGACCCGCGAACTGGTAATTAACGGTACGCCGTACGTCGCGGCGTATGCAATTACCGAAACAGCGGTCCGCATT
CTTCGCGTCCTCCATGGCGCGCAGGAATGGCCAGACACTTTGCCAACGAGCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11110.45 Da
• Isoelectric Point: 5.1751

>AT58941 WP_004669386.1 NZ_CP013556:c825593-825285 [Rhizobium phaseoli]
MAANALVQTRIDAEVRDRASAVLENMGLTVSDAVRILLTRTANEGALPLELFSGSEAHDAWFRTKVLEALNDTRPDVSDD
EVEAQFAKRRAAARLNAGSRKS

Nucleotide

• Length: 309 bp

>AT58941 NZ_CP013556:c825593-825285 [Rhizobium phaseoli]
ATGGCTGCAAATGCACTCGTGCAAACTCGTATTGATGCCGAAGTTCGGGATCGGGCCTCCGCAGTGCTTGAAAACATGGG
GCTTACAGTGTCGGACGCGGTTCGTATCTTGCTCACCCGGACGGCCAACGAGGGTGCGCTGCCACTCGAGCTTTTTTCAG
GCAGCGAGGCCCATGACGCTTGGTTTCGCACCAAGGTGCTTGAAGCGCTGAACGATACTCGACCGGACGTTTCGGATGAT
GAAGTCGAGGCACAATTTGCTAAACGTCGAGCGGCAGCCCGACTTAACGCGGGTTCTCGAAAATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U3CRA1