Detailed information of TA system TA058939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 129682..130256
• Replicon: plasmid pRphaN931b
• Accession: NZ_CP013554
• Organism: Rhizobium phaseoli strain N931

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AMC84_RS23120
• Protein ID: WP_064823502.1
• Coordinates: 129682..130059 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0T6ZM79
• Locus tag: AMC84_RS23125
• Protein ID: WP_017958375.1
• Coordinates: 130056..130256 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC84_RS32525	125518..125766	-	249	WP_150124919.1	ribbon-helix-helix domain-containing protein	-
AMC84_RS23105	125894..126808	+	915	WP_064823500.1	HNH endonuclease	-
AMC84_RS23115	127614..129575	+	1962	WP_064823501.1	elongation factor G	-
AMC84_RS23120	129682..130059	-	378	WP_064823502.1	type II toxin-antitoxin system VapC family toxin	Toxin
AMC84_RS23125	130056..130256	-	201	WP_017958375.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AMC84_RS23130	130425..132542	+	2118	WP_064823503.1	carboxy terminal-processing peptidase	-
AMC84_RS23135	132628..132837	+	210	WP_064823504.1	DUF1778 domain-containing protein	-
AMC84_RS23140	133089..133733	+	645	WP_150124920.1	hypothetical protein	-
AMC84_RS23145	133972..134868	+	897	WP_064823506.1	GGDEF domain-containing protein	-
AMC84_RS23150	134893..135237	-	345	WP_016736588.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..431484	431484

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13943.99 Da
• Isoelectric Point: 6.6034

>T58939 WP_064823502.1 NZ_CP013554:c130059-129682 [Rhizobium phaseoli]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRSNVIAFLAAQRQAFVATHDEVMMMIDRHAVFSM
GIGYTDAHLMASVLLDQRAALWTRDKRLRAAAEKAGASLHTPDNA

Nucleotide

• Length: 378 bp

>T58939 NZ_CP013554:c130059-129682 [Rhizobium phaseoli]
GTGATACTTGCCGACACGTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCTTGTGCCATCCAGCCGTGATCGGAGAGCTCGCACTTGGCAGCCTTCGGGATCGCAGTAACGTGATCGCTTTCC
TGGCGGCCCAGCGCCAAGCGTTCGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGCCACGCCGTTTTCAGCATG
GGCATCGGCTACACCGATGCCCATTTGATGGCTTCGGTTCTCCTCGATCAGCGAGCCGCCTTATGGACGAGAGACAAGCG
TTTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTGCACACGCCCGACAATGCGTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7124.23 Da
• Isoelectric Point: 11.3760

>AT58939 WP_017958375.1 NZ_CP013554:c130256-130056 [Rhizobium phaseoli]
MRSTINLDDTLMERARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT58939 NZ_CP013554:c130256-130056 [Rhizobium phaseoli]
ATGCGATCAACCATCAATCTCGACGACACCCTCATGGAAAGGGCCAGGTCGCTAACCGGCACGAAAGAAACCGCCGCTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCATCGCGCTGGGCGGAACTATGCCGGATG
CGGAGGCAGCTCCACGCCGCCGGAGCGCAGCCGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0T6ZM79