Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 127890..128470 Replicon plasmid pRetR611b
Accession NZ_CP013549
Organism Rhizobium phaseoli strain R611

Toxin (Protein)


Gene name vapC Uniprot ID A0A192TJS5
Locus tag AMC85_RS23935 Protein ID WP_010015189.1
Coordinates 127890..128273 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7X6EWP6
Locus tag AMC85_RS23940 Protein ID WP_064826177.1
Coordinates 128270..128470 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC85_RS23925 123137..125515 + 2379 WP_081277770.1 S8 family peptidase -
AMC85_RS23930 125850..127811 + 1962 WP_064830105.1 elongation factor G -
AMC85_RS23935 127890..128273 - 384 WP_010015189.1 type II toxin-antitoxin system VapC family toxin Toxin
AMC85_RS23940 128270..128470 - 201 WP_064826177.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
AMC85_RS23945 128638..130755 + 2118 WP_064826178.1 carboxy terminal-processing peptidase -
AMC85_RS23950 130869..131051 + 183 WP_081277771.1 DUF1778 domain-containing protein -
AMC85_RS23955 131303..131947 + 645 WP_141489223.1 hypothetical protein -
AMC85_RS23960 132186..133082 + 897 WP_064826181.1 GGDEF domain-containing protein -
AMC85_RS23965 133111..133464 - 354 WP_064826182.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..413457 413457


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14243.32 Da        Isoelectric Point: 6.8449

>T58931 WP_010015189.1 NZ_CP013549:c128273-127890 [Rhizobium phaseoli]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRNNVIAFLAAQREAFVATHDEVMMMIDRHAVFSM
GIGYTDAHLMASVLLDRRAALWTRDKRLRAAAEKAGASLHTPDNARS

Download         Length: 384 bp

>T58931 NZ_CP013549:c128273-127890 [Rhizobium phaseoli]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCCTATGCCATCCGGCCGTGATCGGAGAACTCGCACTTGGCAGCCTTCGGGATCGCAATAACGTCATAGCTTTCC
TGGCGGCCCAGCGCGAAGCGTTCGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGCCACGCCGTTTTCAGCATG
GGCATCGGCTACACAGATGCCCATTTGATGGCTTCAGTTCTACTCGATCGGCGAGCGGCCTTATGGACGAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCCCTTCACACGCCCGACAATGCGCGAAGCTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7299.46 Da        Isoelectric Point: 11.6903

>AT58931 WP_064826177.1 NZ_CP013549:c128470-128270 [Rhizobium phaseoli]
MRSTINLDDTLMERARFLTGTKETATLVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAARK

Download         Length: 201 bp

>AT58931 NZ_CP013549:c128470-128270 [Rhizobium phaseoli]
ATGCGATCAACTATCAATCTCGACGACACCCTCATGGAAAGGGCCAGGTTTCTAACCGGCACGAAAGAAACCGCCACTCT
GGTACGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTTATCGCGCTGGGCGGAACTATGCCGGATG
CGGAGGCAGCCCCACGCCGCCGGAGCGCAGCTCGCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TJS5

Antitoxin

Source ID Structure
AlphaFold DB A0A7X6EWP6

References