Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 920532..921250 Replicon chromosome
Accession NZ_CP013547
Organism Rhizobium phaseoli strain R611

Toxin (Protein)


Gene name vapC Uniprot ID B3PRC0
Locus tag AMC85_RS04440 Protein ID WP_012482827.1
Coordinates 920532..920990 (-) Length 153 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A192T4C6
Locus tag AMC85_RS04445 Protein ID WP_064821400.1
Coordinates 920987..921250 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC85_RS04420 916320..917957 - 1638 WP_012482824.1 chaperonin GroEL -
AMC85_RS04425 918028..918324 - 297 WP_004675403.1 co-chaperone GroES -
AMC85_RS04430 918659..919507 + 849 WP_012482825.1 TIGR01459 family HAD-type hydrolase -
AMC85_RS04435 919522..920505 + 984 WP_064821399.1 bifunctional riboflavin kinase/FAD synthetase -
AMC85_RS04440 920532..920990 - 459 WP_012482827.1 type II toxin-antitoxin system VapC family toxin Toxin
AMC85_RS04445 920987..921250 - 264 WP_064821400.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AMC85_RS04450 921602..924529 + 2928 WP_064823064.1 isoleucine--tRNA ligase -
AMC85_RS04455 924661..925290 + 630 WP_012482830.1 hypothetical protein -
AMC85_RS04460 925562..925999 - 438 WP_064821401.1 nucleoside deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-127)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(7-56)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17015.19 Da        Isoelectric Point: 6.8817

>T58926 WP_012482827.1 NZ_CP013547:c920990-920532 [Rhizobium phaseoli]
VIGWLLDTNVIAALINPNGAPSVKSWAAAQDENQMFISVLTLAEYDKGIENLPEDDRNRYRYIAARDALEERFSQRTLSL
SDAAVRHWGAISGRIKLRTGHAPPVIDTMLAATAIEHNLYLVTRNAKDTRFSGAAVFDPWTHDPNQFPLSRR

Download         Length: 459 bp

>T58926 NZ_CP013547:c920990-920532 [Rhizobium phaseoli]
GTGATCGGATGGCTGCTCGACACCAACGTCATTGCGGCGCTGATCAATCCGAACGGCGCGCCCTCTGTGAAGTCTTGGGC
TGCCGCGCAGGACGAGAACCAGATGTTCATCAGCGTCCTCACCCTCGCAGAATATGACAAAGGCATCGAAAATCTGCCTG
AAGACGACCGAAACCGCTATCGTTATATAGCGGCGCGTGACGCTCTGGAGGAGCGGTTCTCACAGCGTACTCTCTCGCTG
AGCGACGCGGCCGTCAGGCATTGGGGCGCTATTTCAGGGCGCATAAAATTGCGAACCGGACATGCGCCACCGGTCATCGA
CACAATGTTGGCCGCCACCGCCATTGAGCACAATCTCTATCTGGTGACGCGGAACGCAAAGGATACGCGCTTCTCCGGCG
CGGCAGTTTTCGATCCGTGGACACACGATCCGAATCAATTTCCGTTGAGCCGGAGATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10188.70 Da        Isoelectric Point: 8.5940

>AT58926 WP_064821400.1 NZ_CP013547:c921250-920987 [Rhizobium phaseoli]
MRKIVKQSWKLEDAKARFSEVVRRARSEGPQRVTVRGRDSVVIISVEELERLTKAEPRKPLVDFMESLALKEFDLEREAD
YGRDVEL

Download         Length: 264 bp

>AT58926 NZ_CP013547:c921250-920987 [Rhizobium phaseoli]
ATGAGAAAAATTGTAAAGCAAAGCTGGAAGCTCGAGGACGCCAAAGCGCGCTTCAGCGAAGTAGTACGCCGTGCCCGCAG
CGAAGGGCCGCAACGCGTGACAGTGCGCGGCAGAGACAGCGTCGTCATCATCAGCGTCGAAGAACTCGAACGGTTGACGA
AGGCCGAGCCCAGAAAGCCTTTGGTCGATTTCATGGAGAGCCTCGCTCTGAAAGAGTTCGATCTCGAGCGGGAGGCCGAT
TACGGGCGGGATGTCGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M3GMR1

Antitoxin

Source ID Structure
AlphaFold DB A0A192T4C6

References