Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 562154..562689 Replicon plasmid pRphaR620d
Accession NZ_CP013546
Organism Rhizobium phaseoli strain R620

Toxin (Protein)


Gene name relE Uniprot ID B3Q5V1
Locus tag AMC86_RS29180 Protein ID WP_012490004.1
Coordinates 562154..562429 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID F2AEZ7
Locus tag AMC86_RS29185 Protein ID WP_004677393.1
Coordinates 562429..562689 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC86_RS29160 557345..558271 - 927 WP_029531794.1 sugar ABC transporter permease -
AMC86_RS29165 558339..559559 - 1221 WP_037145720.1 sugar ABC transporter substrate-binding protein -
AMC86_RS29170 559609..560931 - 1323 WP_064826428.1 alpha-L-fucosidase -
AMC86_RS29175 561140..562147 + 1008 WP_004677397.1 LacI family transcriptional regulator -
AMC86_RS29180 562154..562429 - 276 WP_012490004.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMC86_RS29185 562429..562689 - 261 WP_004677393.1 ribbon-helix-helix domain-containing protein Antitoxin
AMC86_RS29190 562989..563717 + 729 WP_004677391.1 FadR family transcriptional regulator -
AMC86_RS29195 564008..565021 + 1014 WP_016735851.1 ABC transporter substrate-binding protein -
AMC86_RS29200 565117..565935 + 819 WP_004677388.1 ABC transporter ATP-binding protein -
AMC86_RS29205 565947..566828 + 882 WP_012490008.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gmd 1..1080727 1080727


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10830.44 Da        Isoelectric Point: 9.5543

>T58923 WP_012490004.1 NZ_CP013546:c562429-562154 [Rhizobium phaseoli]
MEIKWTSKAVSDIARLYDFLSPIDRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
VLRLWHTREDR

Download         Length: 276 bp

>T58923 NZ_CP013546:c562429-562154 [Rhizobium phaseoli]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGATCGACCGGCGAGC
TGCGGCCCGAACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GTATTGCGGCTTTGGCATACGCGCGAGGATCGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9686.93 Da        Isoelectric Point: 4.6135

>AT58923 WP_004677393.1 NZ_CP013546:c562689-562429 [Rhizobium phaseoli]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLLR

Download         Length: 261 bp

>AT58923 NZ_CP013546:c562689-562429 [Rhizobium phaseoli]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGACGGTGACGAG
CCGATTTCGTTGCTCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X6IWB5

Antitoxin

Source ID Structure
AlphaFold DB A0A2U3CQR5

References