Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 128777..129384 Replicon plasmid pRphaR620d
Accession NZ_CP013546
Organism Rhizobium phaseoli strain R620

Toxin (Protein)


Gene name hicA Uniprot ID A0A7X6IUV3
Locus tag AMC86_RS27220 Protein ID WP_064826346.1
Coordinates 129130..129384 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7X6IWU0
Locus tag AMC86_RS27215 Protein ID WP_064826345.1
Coordinates 128777..129133 (-) Length 119 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC86_RS33170 125118..125264 - 147 Protein_125 calcium-binding protein -
AMC86_RS27195 125521..126300 - 780 WP_064826342.1 hypothetical protein -
AMC86_RS27205 127579..128034 - 456 WP_064826478.1 hypothetical protein -
AMC86_RS27210 128292..128609 + 318 WP_064826344.1 hypothetical protein -
AMC86_RS27215 128777..129133 - 357 WP_064826345.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AMC86_RS27220 129130..129384 - 255 WP_064826346.1 type II toxin-antitoxin system HicA family toxin Toxin
AMC86_RS27225 129598..130173 - 576 WP_037146207.1 dihydrofolate reductase family protein -
AMC86_RS27230 130472..131233 - 762 WP_064826347.1 class I SAM-dependent methyltransferase -
AMC86_RS27235 131282..131527 - 246 WP_010678589.1 DUF1127 domain-containing protein -
AMC86_RS27240 131623..132414 + 792 WP_064826348.1 helix-turn-helix transcriptional regulator -
AMC86_RS32505 132336..132707 - 372 WP_064830285.1 hypothetical protein -
AMC86_RS27245 132753..133751 - 999 WP_004680351.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gmd 1..1080727 1080727


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-73)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9250.61 Da        Isoelectric Point: 10.2030

>T58922 WP_064826346.1 NZ_CP013546:c129384-129130 [Rhizobium phaseoli]
MNSKQRKTLAAVFKDPVSGTIEWAAIENLLVAAGADLIEGNGSRVKFEKDGIIASFHRPHPAKEAKRYQVRDAREFLMQI
GGTP

Download         Length: 255 bp

>T58922 NZ_CP013546:c129384-129130 [Rhizobium phaseoli]
ATGAACAGCAAGCAGAGAAAGACTCTCGCGGCGGTCTTCAAGGACCCGGTCTCGGGAACCATCGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGGGCCGACCTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAGGACGGGATCATTG
CGAGCTTCCATAGACCACATCCGGCCAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGGAACGCCATGA

Antitoxin


Download         Length: 119 a.a.        Molecular weight: 13271.99 Da        Isoelectric Point: 5.5837

>AT58922 WP_064826345.1 NZ_CP013546:c129133-128777 [Rhizobium phaseoli]
MNIMTYNGYHARIEFDAEDDVFFGRIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLKEAADHFAEARLPA

Download         Length: 357 bp

>AT58922 NZ_CP013546:c129133-128777 [Rhizobium phaseoli]
ATGAACATCATGACCTACAACGGATATCACGCTCGCATCGAATTCGACGCTGAGGACGATGTGTTTTTCGGCAGGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCAGTCGATGACT
ACCTGGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTGAACCAGTGGGCAGAAGAGGTGCTGAAAGAGGC
CGCCGACCATTTTGCCGAGGCGCGCTTGCCGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X6IUV3

Antitoxin

Source ID Structure
AlphaFold DB A0A7X6IWU0

References