Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 280339..281036 Replicon plasmid pRphaR620a
Accession NZ_CP013543
Organism Rhizobium phaseoli strain R620

Toxin (Protein)


Gene name vapC Uniprot ID A0A192TIG6
Locus tag AMC86_RS22635 Protein ID WP_064826047.1
Coordinates 280602..281036 (+) Length 145 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A246S8D2
Locus tag AMC86_RS22630 Protein ID WP_064826046.1
Coordinates 280339..280605 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC86_RS22610 276008..276697 + 690 WP_064826043.1 ABC transporter permease -
AMC86_RS22615 276811..277719 + 909 WP_064826044.1 homocysteine S-methyltransferase family protein -
AMC86_RS22620 277811..278245 - 435 WP_016737028.1 MarR family transcriptional regulator -
AMC86_RS22625 278452..280263 + 1812 WP_064826045.1 chloride channel protein -
AMC86_RS22630 280339..280605 + 267 WP_064826046.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AMC86_RS22635 280602..281036 + 435 WP_064826047.1 type II toxin-antitoxin system VapC family toxin Toxin
AMC86_RS22640 281071..281841 - 771 WP_010020915.1 ABC transporter ATP-binding protein -
AMC86_RS22645 281851..282834 - 984 WP_064826048.1 ABC transporter substrate-binding protein -
AMC86_RS22650 282873..283724 - 852 WP_064826049.1 ABC transporter permease -
AMC86_RS22655 283735..285087 - 1353 WP_064826050.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..356390 356390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-127)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-62)


Sequences

Toxin        


Download         Length: 145 a.a.        Molecular weight: 16116.54 Da        Isoelectric Point: 4.6889

>T58919 WP_064826047.1 NZ_CP013543:280602-281036 [Rhizobium phaseoli]
VRLLLDTNVLSEVTKPNPDMRVLAWLDKLDEDRTFISIVSIAEIRRGVALMDSGRKRDALAEWLARDLPQRFERRIIPVE
EPVALAWGDLMGQAKRSGRGLSSMDGLIAATAVAHDLTLAIRNTRDFEGFGIELVDPWVDPLDP

Download         Length: 435 bp

>T58919 NZ_CP013543:280602-281036 [Rhizobium phaseoli]
GTGAGGCTTCTGCTGGACACCAACGTCCTGTCCGAGGTGACGAAGCCGAACCCTGACATGCGCGTCTTGGCGTGGCTGGA
CAAGCTCGACGAGGATCGCACCTTCATCAGCATCGTGTCGATCGCGGAGATCCGGCGCGGTGTCGCCTTGATGGACAGCG
GACGAAAACGCGACGCGCTGGCCGAATGGCTTGCCCGGGACCTGCCGCAACGCTTCGAGCGTCGCATCATCCCGGTGGAG
GAGCCGGTTGCCTTGGCCTGGGGCGACCTGATGGGCCAGGCGAAGCGGAGCGGTCGCGGCCTTTCCTCAATGGACGGCCT
GATCGCAGCAACCGCGGTTGCTCACGATCTCACTCTGGCTATACGTAACACCAGAGATTTCGAGGGATTCGGGATCGAAC
TCGTTGATCCCTGGGTCGATCCTCTCGATCCCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9848.06 Da        Isoelectric Point: 6.3615

>AT58919 WP_064826046.1 NZ_CP013543:280339-280605 [Rhizobium phaseoli]
MQPSRQGDESWTVASAKAKLSEVIERAQSAPQTITRNGKPSVVVVSAEEWQRKTARKGTLVEFLMSSPLRGADLDLERQR
DEPRDPEL

Download         Length: 267 bp

>AT58919 NZ_CP013543:280339-280605 [Rhizobium phaseoli]
ATGCAGCCATCACGGCAAGGCGATGAAAGCTGGACCGTTGCCAGCGCAAAGGCAAAGCTTTCCGAGGTTATCGAACGCGC
GCAATCCGCGCCGCAGACGATTACCCGGAACGGCAAGCCGAGCGTCGTCGTCGTCTCCGCCGAGGAATGGCAGCGCAAGA
CGGCGCGCAAGGGTACGCTTGTCGAGTTTCTGATGAGTTCGCCGCTGCGCGGCGCTGATCTGGATCTCGAGCGTCAACGC
GACGAACCGCGTGATCCTGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192TIG6

Antitoxin

Source ID Structure
AlphaFold DB A0A246S8D2

References