Detailed information of TA system TA058918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2939834..2940507
• Replicon: chromosome
• Accession: NZ_CP013542
• Organism: Rhizobium phaseoli strain R620

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AMC86_RS14355
• Protein ID: WP_064825467.1
• Coordinates: 2939834..2940247 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B3PTS5
• Locus tag: AMC86_RS14360
• Protein ID: WP_012484474.1
• Coordinates: 2940244..2940507 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC86_RS14345	2937302..2938510	-	1209	WP_012484471.1	efflux RND transporter periplasmic adaptor subunit	-
AMC86_RS14350	2938803..2939711	-	909	WP_064825466.1	adenylate/guanylate cyclase domain-containing protein	-
AMC86_RS14355	2939834..2940247	-	414	WP_064825467.1	type II toxin-antitoxin system VapC family toxin	Toxin
AMC86_RS14360	2940244..2940507	-	264	WP_012484474.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AMC86_RS14365	2940586..2942136	-	1551	WP_016733577.1	SpoVR family protein	-
AMC86_RS14370	2942133..2943407	-	1275	WP_012484476.1	YeaH/YhbH family protein	-
AMC86_RS14375	2943420..2945363	-	1944	WP_004673346.1	PrkA family serine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (9-57)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15175.67 Da
• Isoelectric Point: 7.2781

>T58918 WP_064825467.1 NZ_CP013542:c2940247-2939834 [Rhizobium phaseoli]
MTAWMLDTNIASHVIKGDRPEILRRLAALPMDGIVISSITEGELLYGLAKRDYPKALSERVRQFLVRVDVLPWDHGVMRA
YGDLRAACEAKGTILAPLDMMIAAHAVATAATLVTRDKAFARISEPLKVDDWTSERS

Nucleotide

• Length: 414 bp

>T58918 NZ_CP013542:c2940247-2939834 [Rhizobium phaseoli]
ATGACGGCATGGATGCTCGACACGAATATCGCCAGTCATGTCATCAAGGGCGATCGGCCGGAGATACTTAGAAGACTGGC
GGCGCTTCCGATGGACGGGATCGTCATCTCATCGATCACGGAAGGGGAATTGCTCTATGGTCTTGCCAAGCGAGACTATC
CGAAGGCGCTATCCGAGCGAGTAAGGCAATTCCTCGTTAGGGTCGATGTTCTCCCCTGGGATCACGGCGTCATGCGAGCC
TATGGCGACCTGCGCGCCGCCTGTGAAGCCAAAGGCACGATTCTGGCGCCCCTGGATATGATGATTGCCGCTCATGCCGT
CGCGACCGCGGCAACCCTCGTCACGCGTGACAAGGCTTTTGCTCGCATTTCCGAGCCTTTAAAGGTCGATGACTGGACGA
GCGAAAGAAGCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10024.18 Da
• Isoelectric Point: 5.1847

>AT58918 WP_012484474.1 NZ_CP013542:c2940507-2940244 [Rhizobium phaseoli]
MATKTAKIFTTGRSQAVRLPAEFRFEESEVFVRRDPKTGDVILSRKPDSWDGLFELHRKDPIPDDFLGAADRSQPSQDRD
PFEGWKE

Nucleotide

• Length: 264 bp

>AT58918 NZ_CP013542:c2940507-2940244 [Rhizobium phaseoli]
ATGGCCACGAAAACGGCTAAGATTTTTACGACCGGCCGCAGCCAGGCGGTGAGACTCCCCGCGGAGTTCCGCTTTGAAGA
GAGCGAAGTGTTCGTTCGTCGTGATCCGAAGACCGGTGATGTCATATTATCGCGCAAGCCGGATTCATGGGATGGCCTTT
TCGAACTCCATCGGAAGGATCCGATTCCGGACGATTTCCTTGGGGCGGCCGATCGCAGCCAGCCTTCGCAAGATCGTGAT
CCGTTCGAAGGTTGGAAGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0M3GIB4