Detailed information of TA system TA058913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 564458..564993
• Replicon: plasmid pRphaR630d
• Accession: NZ_CP013541
• Organism: Rhizobium phaseoli strain R630

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC87_RS29315
• Protein ID: WP_064829590.1
• Coordinates: 564458..564733 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2AEZ7
• Locus tag: AMC87_RS29320
• Protein ID: WP_004677393.1
• Coordinates: 564733..564993 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC87_RS29295	559649..560575	-	927	WP_029531794.1	sugar ABC transporter permease	-
AMC87_RS29300	560643..561863	-	1221	WP_037145720.1	sugar ABC transporter substrate-binding protein	-
AMC87_RS29305	561913..563235	-	1323	WP_064829589.1	alpha-L-fucosidase	-
AMC87_RS29310	563444..564451	+	1008	WP_004677397.1	LacI family transcriptional regulator	-
AMC87_RS29315	564458..564733	-	276	WP_064829590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMC87_RS29320	564733..564993	-	261	WP_004677393.1	ribbon-helix-helix domain-containing protein	Antitoxin
AMC87_RS29325	565293..566021	+	729	WP_004677391.1	FadR family transcriptional regulator	-
AMC87_RS29330	566312..567325	+	1014	WP_016735851.1	ABC transporter substrate-binding protein	-
AMC87_RS29335	567421..568239	+	819	WP_004677388.1	ABC transporter ATP-binding protein	-
AMC87_RS29340	568251..569132	+	882	WP_012490008.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gmd	1..1144409	1144409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10829.45 Da
• Isoelectric Point: 9.9706

>T58913 WP_064829590.1 NZ_CP013541:c564733-564458 [Rhizobium phaseoli]
MEIKWTSKAVSDIARLYDFLSPINRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T58913 NZ_CP013541:c564733-564458 [Rhizobium phaseoli]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGATCAACCGGCGAGC
TGCGGCCCGAACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GTATTGCGGCTTTGGCATACGCGCGAGGATCGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9686.93 Da
• Isoelectric Point: 4.6135

>AT58913 WP_004677393.1 NZ_CP013541:c564993-564733 [Rhizobium phaseoli]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLLR

Nucleotide

• Length: 261 bp

>AT58913 NZ_CP013541:c564993-564733 [Rhizobium phaseoli]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGACGGTGACGAG
CCGATTTCGTTGCTCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U3CQR5