Detailed information of TA system TA058911

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 131777..132357
• Replicon: plasmid pRphaR630b
• Accession: NZ_CP013539
• Organism: Rhizobium phaseoli strain R630

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AMC87_RS23410
• Protein ID: WP_064829263.1
• Coordinates: 131777..132160 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AMC87_RS23415
• Protein ID: WP_064829264.1
• Coordinates: 132157..132357 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC87_RS23400	127988..129256	-	1269	WP_064829262.1	phospholipase D family protein	-
AMC87_RS23405	129736..131697	+	1962	WP_064829437.1	elongation factor G	-
AMC87_RS23410	131777..132160	-	384	WP_064829263.1	type II toxin-antitoxin system VapC family toxin	Toxin
AMC87_RS23415	132157..132357	-	201	WP_064829264.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AMC87_RS23420	132692..134809	+	2118	WP_064829265.1	carboxy terminal-processing peptidase	-
AMC87_RS23425	134923..135105	+	183	WP_009999637.1	DUF1778 domain-containing protein	-
AMC87_RS23430	135258..136001	+	744	WP_150129099.1	hypothetical protein	-
AMC87_RS23435	136242..137138	+	897	WP_016736587.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..421824	421824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14181.28 Da
• Isoelectric Point: 7.1300

>T58911 WP_064829263.1 NZ_CP013539:c132160-131777 [Rhizobium phaseoli]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRSNVIAFLAAQRQAFVATHDEVMMMIDRHAIFSM
GIGYTDAHLIASVLLDRRAALWTRDKRLRAAAEKAGASLHAPDNARS

Nucleotide

• Length: 384 bp

>T58911 NZ_CP013539:c132160-131777 [Rhizobium phaseoli]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCTTATGCCATCCAGCCGTGATCGGAGAGCTCGCACTTGGCAGCCTTCGGGATCGCAGTAACGTCATAGCTTTCC
TGGCGGCCCAGCGCCAAGCGTTCGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGTCACGCCATTTTCAGCATG
GGCATTGGCTACACAGATGCCCATTTGATCGCTTCGGTTCTACTCGATCGGCGAGCGGCCTTATGGACGAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTTCACGCGCCCGACAATGCGCGAAGCTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7154.25 Da
• Isoelectric Point: 10.9613

>AT58911 WP_064829264.1 NZ_CP013539:c132357-132157 [Rhizobium phaseoli]
MRSTINLDDTLMEWARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT58911 NZ_CP013539:c132357-132157 [Rhizobium phaseoli]
ATGCGATCAACCATCAATCTCGACGACACCCTCATGGAATGGGCCAGGTCCCTAACCGGCACGAAAGAAACCGCCGCTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCATCGCGCTGGGCGGAACTATGCCGGATG
CGGAGGCAGCTCCACGCCGCCGGAGCGCAGCCGCCAAGTGA