Detailed information of TA system TA058907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 871227..871825
• Replicon: plasmid pRphaR650d
• Accession: NZ_CP013536
• Organism: Rhizobium phaseoli strain R650

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC88_RS32900
• Protein ID: WP_081279432.1
• Coordinates: 871227..871520 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7X6J1Z1
• Locus tag: AMC88_RS31205
• Protein ID: WP_064820408.1
• Coordinates: 871517..871825 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC88_RS31185	866543..867304	-	762	WP_064820405.1	SDR family oxidoreductase	-
AMC88_RS31190	867444..868307	+	864	WP_037144412.1	LysR family transcriptional regulator	-
AMC88_RS31195	868318..868737	-	420	Protein_799	error-prone DNA polymerase	-
AMC88_RS33935	870040..870342	-	303	WP_185185227.1	diguanylate cyclase	-
AMC88_RS33940	870339..870713	-	375	WP_190311520.1	hypothetical protein	-
AMC88_RS32900	871227..871520	-	294	WP_081279432.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
AMC88_RS31205	871517..871825	-	309	WP_064820408.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AMC88_RS31210	872141..872929	-	789	WP_081279433.1	NAD(P)-dependent alcohol dehydrogenase	-
AMC88_RS31215	873114..873497	-	384	WP_064820409.1	DoxX family protein	-
AMC88_RS31220	873512..874360	-	849	WP_064820410.1	SDR family oxidoreductase	-
AMC88_RS31225	874496..874897	+	402	WP_064820580.1	helix-turn-helix transcriptional regulator	-
AMC88_RS31230	874938..875385	-	448	Protein_808	transposase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..990934	990934
	-	flank	IS/Tn	-	-	875042..875254	212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10624.19 Da
• Isoelectric Point: 5.7996

>T58907 WP_081279432.1 NZ_CP013536:c871520-871227 [Rhizobium phaseoli]
VKLTWSVFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLIDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDTLPTS

Nucleotide

• Length: 294 bp

>T58907 NZ_CP013536:c871520-871227 [Rhizobium phaseoli]
GTGAAGCTTACCTGGTCTGTCTTCGCATTATCTGACCGCGACGCCATATTTACGTACATAGAAGCGGAAAATCCGTCAGC
CGCTATTCTGGTCGACGAACGGATCGTTGCTGCCGTTCGTCGGTTGATAGATTTTCCCGCAAGCGGTCGTGTTGGCAGAA
TTGCTGGAACCCGCGAACTGGTAATTAACGGTACGCCGTACGTCGCGGCTTATGCAATTACCGAAACAGCAGTCCGTATT
CTTCGCGTCCTCCATGGCGCGCAGGAATGGCCAGACACTTTGCCAACGAGCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11013.38 Da
• Isoelectric Point: 6.4721

>AT58907 WP_064820408.1 NZ_CP013536:c871825-871517 [Rhizobium phaseoli]
MAANALVQTRIDAEVRDRASAVLENMGLTVSDAVRILLTRTANEGALPLELLSGSEAHDAWFRTKVLEALNDTRPDVSDN
EAEAHFAARRAAARLKAGSRKS

Nucleotide

• Length: 309 bp

>AT58907 NZ_CP013536:c871825-871517 [Rhizobium phaseoli]
ATGGCTGCAAATGCACTCGTGCAAACTCGTATTGATGCCGAAGTCCGGGATCGGGCCTCCGCAGTGCTTGAAAACATGGG
GCTTACAGTATCGGACGCGGTTCGTATCTTGCTCACCCGGACGGCCAATGAGGGTGCGCTGCCACTCGAGCTTCTCTCAG
GCAGCGAGGCGCATGACGCGTGGTTTCGCACCAAGGTGCTTGAAGCGCTGAACGATACTCGACCAGACGTCTCGGATAAC
GAAGCCGAGGCACATTTTGCCGCACGTCGAGCGGCAGCCCGACTTAAAGCGGGTTCTCGAAAATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X6J1Z1