Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 784617..785224 Replicon plasmid pRphaR650d
Accession NZ_CP013536
Organism Rhizobium phaseoli strain R650

Toxin (Protein)


Gene name hicA Uniprot ID A0A7X6IUV3
Locus tag AMC88_RS30805 Protein ID WP_064826346.1
Coordinates 784617..784871 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7X6IWU0
Locus tag AMC88_RS30810 Protein ID WP_064826345.1
Coordinates 784868..785224 (+) Length 119 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC88_RS30775 780250..781248 + 999 WP_004680351.1 LacI family DNA-binding transcriptional regulator -
AMC88_RS30780 781294..781665 + 372 WP_064830285.1 hypothetical protein -
AMC88_RS30785 781587..782378 - 792 WP_064826348.1 helix-turn-helix transcriptional regulator -
AMC88_RS30790 782474..782719 + 246 WP_010678589.1 DUF1127 domain-containing protein -
AMC88_RS30795 782768..783529 + 762 WP_064826347.1 class I SAM-dependent methyltransferase -
AMC88_RS30800 783828..784403 + 576 WP_037146207.1 dihydrofolate reductase family protein -
AMC88_RS30805 784617..784871 + 255 WP_064826346.1 type II toxin-antitoxin system HicA family toxin Toxin
AMC88_RS30810 784868..785224 + 357 WP_064826345.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AMC88_RS30815 785392..785709 - 318 WP_064826344.1 hypothetical protein -
AMC88_RS30820 785967..786422 + 456 WP_064826478.1 hypothetical protein -
AMC88_RS30830 787701..788480 + 780 WP_064826342.1 hypothetical protein -
AMC88_RS33475 788737..788883 + 147 Protein_724 calcium-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - gmd 1..990934 990934


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-73)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9250.61 Da        Isoelectric Point: 10.2030

>T58905 WP_064826346.1 NZ_CP013536:784617-784871 [Rhizobium phaseoli]
MNSKQRKTLAAVFKDPVSGTIEWAAIENLLVAAGADLIEGNGSRVKFEKDGIIASFHRPHPAKEAKRYQVRDAREFLMQI
GGTP

Download         Length: 255 bp

>T58905 NZ_CP013536:784617-784871 [Rhizobium phaseoli]
ATGAACAGCAAGCAGAGAAAGACTCTCGCGGCGGTCTTCAAGGACCCGGTCTCGGGAACCATCGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGGGCCGACCTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAGGACGGGATCATTG
CGAGCTTCCATAGACCACATCCGGCCAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGGAACGCCATGA

Antitoxin


Download         Length: 119 a.a.        Molecular weight: 13271.99 Da        Isoelectric Point: 5.5837

>AT58905 WP_064826345.1 NZ_CP013536:784868-785224 [Rhizobium phaseoli]
MNIMTYNGYHARIEFDAEDDVFFGRIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLKEAADHFAEARLPA

Download         Length: 357 bp

>AT58905 NZ_CP013536:784868-785224 [Rhizobium phaseoli]
ATGAACATCATGACCTACAACGGATATCACGCTCGCATCGAATTCGACGCTGAGGACGATGTGTTTTTCGGCAGGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCAGTCGATGACT
ACCTGGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTGAACCAGTGGGCAGAAGAGGTGCTGAAAGAGGC
CGCCGACCATTTTGCCGAGGCGCGCTTGCCGGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X6IUV3

Antitoxin

Source ID Structure
AlphaFold DB A0A7X6IWU0

References