Detailed information of TA system TA058897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 836374..836972
• Replicon: plasmid pRphaR723d
• Accession: NZ_CP013531
• Organism: Rhizobium phaseoli strain R723

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC89_RS33165
• Protein ID: WP_081279432.1
• Coordinates: 836374..836667 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7X6J1Z1
• Locus tag: AMC89_RS30775
• Protein ID: WP_064820408.1
• Coordinates: 836664..836972 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC89_RS30755	831690..832451	-	762	WP_064820405.1	SDR family oxidoreductase	-
AMC89_RS30760	832591..833454	+	864	WP_064820406.1	LysR family transcriptional regulator	-
AMC89_RS30765	833465..833884	-	420	Protein_773	error-prone DNA polymerase	-
AMC89_RS34240	835187..835489	-	303	WP_185185227.1	diguanylate cyclase	-
AMC89_RS34245	835486..835860	-	375	WP_185185226.1	hypothetical protein	-
AMC89_RS33165	836374..836667	-	294	WP_081279432.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
AMC89_RS30775	836664..836972	-	309	WP_064820408.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AMC89_RS30780	837288..838076	-	789	WP_081279433.1	NAD(P)-dependent alcohol dehydrogenase	-
AMC89_RS30785	838261..838644	-	384	WP_064820409.1	DoxX family protein	-
AMC89_RS30790	838659..839507	-	849	WP_064820410.1	SDR family oxidoreductase	-
AMC89_RS30795	839643..840044	+	402	WP_064820580.1	helix-turn-helix transcriptional regulator	-
AMC89_RS30800	840085..840532	-	448	Protein_782	transposase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gmd	1..1074795	1074795
	-	flank	IS/Tn	-	-	840189..840401	212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10624.19 Da
• Isoelectric Point: 5.7996

>T58897 WP_081279432.1 NZ_CP013531:c836667-836374 [Rhizobium phaseoli]
VKLTWSVFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLIDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDTLPTS

Nucleotide

• Length: 294 bp

>T58897 NZ_CP013531:c836667-836374 [Rhizobium phaseoli]
GTGAAGCTTACCTGGTCTGTCTTCGCATTATCTGACCGCGACGCCATATTTACGTACATAGAAGCGGAAAATCCGTCAGC
CGCTATTCTGGTCGACGAACGGATCGTTGCTGCCGTTCGTCGGTTGATAGATTTTCCCGCAAGCGGTCGTGTTGGCAGAA
TTGCTGGAACCCGCGAACTGGTAATTAACGGTACGCCGTACGTCGCGGCTTATGCAATTACCGAAACAGCAGTCCGTATT
CTTCGCGTCCTCCATGGCGCGCAGGAATGGCCAGACACTTTGCCAACGAGCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11013.38 Da
• Isoelectric Point: 6.4721

>AT58897 WP_064820408.1 NZ_CP013531:c836972-836664 [Rhizobium phaseoli]
MAANALVQTRIDAEVRDRASAVLENMGLTVSDAVRILLTRTANEGALPLELLSGSEAHDAWFRTKVLEALNDTRPDVSDN
EAEAHFAARRAAARLKAGSRKS

Nucleotide

• Length: 309 bp

>AT58897 NZ_CP013531:c836972-836664 [Rhizobium phaseoli]
ATGGCTGCAAATGCACTCGTGCAAACTCGTATTGATGCCGAAGTCCGGGATCGGGCCTCCGCAGTGCTTGAAAACATGGG
GCTTACAGTATCGGACGCGGTTCGTATCTTGCTCACCCGGACGGCCAATGAGGGTGCGCTGCCACTCGAGCTTCTCTCAG
GCAGCGAGGCGCATGACGCGTGGTTTCGCACCAAGGTGCTTGAAGCGCTGAACGATACTCGACCAGACGTCTCGGATAAC
GAAGCCGAGGCACATTTTGCCGCACGTCGAGCGGCAGCCCGACTTAAAGCGGGTTCTCGAAAATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X6J1Z1