Detailed information of TA system TA058893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 129059..129639
• Replicon: plasmid pRphaR723b
• Accession: NZ_CP013529
• Organism: Rhizobium phaseoli strain R723

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AMC89_RS23620
• Protein ID: WP_064819821.1
• Coordinates: 129059..129442 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7W6MIA3
• Locus tag: AMC89_RS23625
• Protein ID: WP_016736583.1
• Coordinates: 129439..129639 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC89_RS23605	125109..126308	+	1200	WP_064819820.1	mandelate racemase/muconate lactonizing enzyme family protein	-
AMC89_RS23610	126524..126823	-	300	WP_010001186.1	hypothetical protein	-
AMC89_RS23615	127018..128979	+	1962	WP_064819985.1	elongation factor G	-
AMC89_RS23620	129059..129442	-	384	WP_064819821.1	type II toxin-antitoxin system VapC family toxin	Toxin
AMC89_RS23625	129439..129639	-	201	WP_016736583.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AMC89_RS23630	129967..132084	+	2118	WP_064819822.1	carboxy terminal-processing peptidase	-
AMC89_RS23635	132237..132482	+	246	WP_064819986.1	DUF1778 domain-containing protein	-
AMC89_RS23640	132479..132973	+	495	WP_064819823.1	GNAT family N-acetyltransferase	-
AMC89_RS23645	133102..133998	+	897	WP_016736587.1	GGDEF domain-containing protein	-
AMC89_RS23650	134023..134367	-	345	WP_016736588.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..427855	427855

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14229.34 Da
• Isoelectric Point: 7.1300

>T58893 WP_064819821.1 NZ_CP013529:c129442-129059 [Rhizobium phaseoli]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRSNVIAFLAAQRQAFVATHDEVMMMIDRHAIFSM
GIGYTDAHLMASVLLDRRAALWTRDKRLRAAAEKAGASLHTPDNARS

Nucleotide

• Length: 384 bp

>T58893 NZ_CP013529:c129442-129059 [Rhizobium phaseoli]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCTTGTGCCATCCAGCCGTGATCGGAGAGCTCGCACTTGGCAGCCTTCGGGATCGCAGTAACGTGATCGCTTTCC
TGGCGGCCCAGCGCCAAGCGTTCGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGCCACGCCATTTTCAGCATG
GGCATTGGCTACACAGATGCCCATTTGATGGCTTCGGTTCTACTCGATCGGCGAGCGGCCTTATGGACGAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCCCTTCACACGCCCGACAATGCGCGAAGCTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7154.26 Da
• Isoelectric Point: 11.3760

>AT58893 WP_016736583.1 NZ_CP013529:c129639-129439 [Rhizobium phaseoli]
MRSTINLDDTLMERARSLTGTKETATLVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT58893 NZ_CP013529:c129639-129439 [Rhizobium phaseoli]
ATGCGATCAACCATCAATCTCGACGACACCCTTATGGAAAGGGCGAGGTCCCTAACCGGCACGAAAGAAACCGCCACTCT
GGTCCGCCAAGCGCTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCATCGCGCTGGGCGGAACTATGCCGGATG
CGGAGGCAGCTCCACGCCGCCGGAGCGCAGCCGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W6MIA3