Detailed information of TA system TA058892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 92200..92993
• Replicon: plasmid pRphaR723a
• Accession: NZ_CP013528
• Organism: Rhizobium phaseoli strain R723

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: AMC89_RS21830
• Protein ID: WP_037147374.1
• Coordinates: 92200..92709 (-)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F2ACB7
• Locus tag: AMC89_RS21835
• Protein ID: WP_004675906.1
• Coordinates: 92709..92993 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC89_RS21805	88384..89994	-	1611	WP_064819567.1	methylcrotonoyl-CoA carboxylase	-
AMC89_RS21810	89991..91136	-	1146	WP_064819568.1	isovaleryl-CoA dehydrogenase	-
AMC89_RS21815	91243..91503	+	261	WP_004675900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AMC89_RS21820	91567..91862	-	296	Protein_87	type II toxin-antitoxin system RelE/ParE family toxin	-
AMC89_RS21825	91859..92128	-	270	WP_037116230.1	type II toxin-antitoxin system ParD family antitoxin	-
AMC89_RS21830	92200..92709	-	510	WP_037147374.1	GNAT family N-acetyltransferase	Toxin
AMC89_RS21835	92709..92993	-	285	WP_004675906.1	DUF1778 domain-containing protein	Antitoxin
AMC89_RS21840	93234..94241	-	1008	WP_064819569.1	AraC family transcriptional regulator	-
AMC89_RS21845	94378..95145	+	768	WP_064819570.1	SDR family NAD(P)-dependent oxidoreductase	-
AMC89_RS21850	95166..96353	+	1188	WP_064819571.1	acetyl-CoA C-acyltransferase	-
AMC89_RS21855	96370..97497	+	1128	WP_064819572.1	acyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..349793	349793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-80)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18183.76 Da
• Isoelectric Point: 7.3830

>T58892 WP_037147374.1 NZ_CP013528:c92709-92200 [Rhizobium phaseoli]
MLSAPALLGEAHDLRLFDSGHDSLDEWLRRRARANQVSGASRTYVVCEDERVVGYYCLSSGALAVAEAPGSIRRNMPDPV
PMAVLGRLAIDRDWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDGAKAFYEHYGFTATPTNPMTLVLSLKPRED
LKQSPSAAS

Nucleotide

• Length: 510 bp

>T58892 NZ_CP013528:c92709-92200 [Rhizobium phaseoli]
ATGTTATCAGCACCGGCGCTTCTCGGCGAAGCGCACGATCTCCGGCTCTTCGACAGCGGGCACGACAGTCTCGATGAATG
GCTGCGCCGCCGGGCGCGGGCTAATCAGGTCAGCGGCGCATCGCGAACCTATGTGGTTTGCGAGGATGAACGCGTTGTCG
GATATTATTGCCTGTCATCGGGAGCGCTCGCGGTTGCCGAAGCGCCGGGATCAATCCGGCGCAATATGCCGGACCCTGTT
CCCATGGCGGTGCTCGGCCGTCTGGCCATAGACCGCGACTGGCAGGGAAAGGGAATCGGTGCCGCGCTGTTGCAAGACGC
AGTGCTGCGCACCGGACAGGCTGCGCATATTATGGGAATTCGCGGCCTTCTGGTTCATGCGATCTCCGACGGAGCCAAGG
CCTTTTACGAGCACTACGGATTTACGGCGACGCCCACGAATCCCATGACGTTGGTTTTGTCGCTGAAACCGCGGGAAGAC
TTGAAGCAGTCGCCCTCAGCCGCCTCCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10545.96 Da
• Isoelectric Point: 8.5686

>AT58892 WP_004675906.1 NZ_CP013528:c92993-92709 [Rhizobium phaseoli]
MAGKNATDTQKRPVNLKVREDIRVLIDRAARARGKTRSDFMIDAARRAAEDALLDQTLVQVDPDSYEHYLAVLDQPPSGE
GFARLMNAPKPWQP

Nucleotide

• Length: 285 bp

>AT58892 NZ_CP013528:c92993-92709 [Rhizobium phaseoli]
ATGGCTGGCAAAAACGCAACCGATACCCAAAAACGCCCTGTCAACCTGAAGGTCCGTGAGGACATTCGCGTCCTGATCGA
CCGTGCCGCCAGGGCCCGCGGCAAAACCCGGTCGGATTTCATGATCGATGCTGCCCGGCGCGCCGCCGAAGACGCGCTTC
TCGATCAGACATTGGTGCAGGTCGATCCCGACAGCTATGAACACTATCTGGCCGTGCTCGACCAGCCGCCAAGCGGCGAA
GGCTTCGCACGGCTGATGAACGCTCCCAAACCTTGGCAGCCGTAA

Structures

Antitoxin

• AlphaFold DB: F2ACB7