Detailed information of TA system TA058887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 981545..982143
• Replicon: plasmid pRphaR744d
• Accession: NZ_CP013526
• Organism: Rhizobium phaseoli strain R744

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMC90_RS32460
• Protein ID: WP_080574712.1
• Coordinates: 981545..981838 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2A2S3
• Locus tag: AMC90_RS30505
• Protein ID: WP_004669386.1
• Coordinates: 981835..982143 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC90_RS30470	976739..978205	+	1467	WP_064844798.1	TolC family protein	-
AMC90_RS30475	978220..979566	+	1347	WP_064844800.1	copper oxidase	-
AMC90_RS30480	979601..980080	+	480	WP_010005502.1	cupredoxin domain-containing protein	-
AMC90_RS30485	980103..980390	+	288	WP_037144323.1	copper-binding protein	-
AMC90_RS30490	980446..980907	+	462	WP_064844802.1	DUF411 domain-containing protein	-
AMC90_RS30495	980943..981314	-	372	WP_037144318.1	hypothetical protein	-
AMC90_RS32460	981545..981838	-	294	WP_080574712.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
AMC90_RS30505	981835..982143	-	309	WP_004669386.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
AMC90_RS30510	982357..982738	-	382	Protein_912	DAK2 domain-containing protein	-
AMC90_RS33475	982760..983206	+	447	WP_185171516.1	hypothetical protein	-
AMC90_RS30515	983379..984281	-	903	WP_064844804.1	NADP-dependent oxidoreductase	-
AMC90_RS30520	984361..984759	-	399	WP_064844806.1	DoxX family protein	-
AMC90_RS30525	984788..985168	-	381	WP_064844808.1	glutathione S-transferase N-terminal domain-containing protein	-
AMC90_RS30530	985165..986010	-	846	WP_064844810.1	dioxygenase	-
AMC90_RS30535	986130..987047	+	918	WP_185171519.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gmd	1..1195489	1195489

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10596.14 Da
• Isoelectric Point: 5.7996

>T58887 WP_080574712.1 NZ_CP013526:c981838-981545 [Rhizobium phaseoli]
VKLTWSAFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLIDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDTLPTS

Nucleotide

• Length: 294 bp

>T58887 NZ_CP013526:c981838-981545 [Rhizobium phaseoli]
GTGAAGCTTACCTGGTCTGCCTTCGCATTATCGGATCGCGACGCCATATTCACATACATAGAAGCGGAAAATCCGTCAGC
CGCTATTCTGGTCGACGAACGGATCGTTGCTGCCGTTCGTCGGTTGATAGATTTTCCCGCGAGCGGTCGTGTCGGCAGAA
TTGCTGGGACCCGCGAACTGGTAATTAACGGTACGCCGTACGTCGCGGCGTATGCAATTACCGAAACAGCGGTCCGCATT
CTTCGCGTCCTCCATGGCGCGCAGGAATGGCCAGACACTTTGCCAACGAGCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11110.45 Da
• Isoelectric Point: 5.1751

>AT58887 WP_004669386.1 NZ_CP013526:c982143-981835 [Rhizobium phaseoli]
MAANALVQTRIDAEVRDRASAVLENMGLTVSDAVRILLTRTANEGALPLELFSGSEAHDAWFRTKVLEALNDTRPDVSDD
EVEAQFAKRRAAARLNAGSRKS

Nucleotide

• Length: 309 bp

>AT58887 NZ_CP013526:c982143-981835 [Rhizobium phaseoli]
ATGGCTGCAAATGCACTCGTGCAAACTCGTATTGATGCCGAAGTTCGGGATCGGGCCTCCGCAGTGCTTGAAAACATGGG
GCTTACAGTGTCGGACGCGGTTCGTATCTTGCTCACCCGGACGGCCAACGAGGGTGCGCTGCCACTCGAGCTTTTTTCAG
GCAGCGAGGCCCATGACGCTTGGTTTCGCACCAAGGTGCTTGAAGCGCTGAACGATACTCGACCGGACGTTTCGGATGAT
GAAGTCGAGGCACAATTTGCTAAACGTCGAGCGGCAGCCCGACTTAACGCGGGTTCTCGAAAATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U3CRA1