Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 551784..552319 Replicon plasmid pRphaR744d
Accession NZ_CP013526
Organism Rhizobium phaseoli strain R744

Toxin (Protein)


Gene name relE Uniprot ID B3Q5V1
Locus tag AMC90_RS28520 Protein ID WP_012490004.1
Coordinates 551784..552059 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID F2AEZ7
Locus tag AMC90_RS28525 Protein ID WP_004677393.1
Coordinates 552059..552319 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMC90_RS28500 546975..547901 - 927 WP_029531794.1 sugar ABC transporter permease -
AMC90_RS28505 547969..549189 - 1221 WP_037145720.1 sugar ABC transporter substrate-binding protein -
AMC90_RS28510 549239..550561 - 1323 WP_064826428.1 alpha-L-fucosidase -
AMC90_RS28515 550770..551777 + 1008 WP_004677397.1 LacI family transcriptional regulator -
AMC90_RS28520 551784..552059 - 276 WP_012490004.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMC90_RS28525 552059..552319 - 261 WP_004677393.1 ribbon-helix-helix domain-containing protein Antitoxin
AMC90_RS28530 552619..553347 + 729 WP_064844466.1 FadR family transcriptional regulator -
AMC90_RS28535 553638..554651 + 1014 WP_016735851.1 ABC transporter substrate-binding protein -
AMC90_RS28540 554747..555565 + 819 WP_004677388.1 ABC transporter ATP-binding protein -
AMC90_RS28545 555577..556458 + 882 WP_064844467.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gmd 1..1195489 1195489


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10830.44 Da        Isoelectric Point: 9.5543

>T58886 WP_012490004.1 NZ_CP013526:c552059-551784 [Rhizobium phaseoli]
MEIKWTSKAVSDIARLYDFLSPIDRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
VLRLWHTREDR

Download         Length: 276 bp

>T58886 NZ_CP013526:c552059-551784 [Rhizobium phaseoli]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGATCGACCGGCGAGC
TGCGGCCCGAACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GTATTGCGGCTTTGGCATACGCGCGAGGATCGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9686.93 Da        Isoelectric Point: 4.6135

>AT58886 WP_004677393.1 NZ_CP013526:c552319-552059 [Rhizobium phaseoli]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLLR

Download         Length: 261 bp

>AT58886 NZ_CP013526:c552319-552059 [Rhizobium phaseoli]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGACGGTGACGAG
CCGATTTCGTTGCTCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X6IWB5

Antitoxin

Source ID Structure
AlphaFold DB A0A2U3CQR5

References