Detailed information of TA system TA058884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 127441..128021
• Replicon: plasmid pRphaR744c
• Accession: NZ_CP013525
• Organism: Rhizobium phaseoli strain R744

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AMC90_RS24675
• Protein ID: WP_064844257.1
• Coordinates: 127441..127824 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7W6MIA3
• Locus tag: AMC90_RS24680
• Protein ID: WP_016736583.1
• Coordinates: 127821..128021 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMC90_RS24665	123733..124485	-	753	WP_064844254.1	DUF4157 domain-containing protein	-
AMC90_RS24670	125400..127361	+	1962	WP_064844255.1	elongation factor G	-
AMC90_RS24675	127441..127824	-	384	WP_064844257.1	type II toxin-antitoxin system VapC family toxin	Toxin
AMC90_RS24680	127821..128021	-	201	WP_016736583.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AMC90_RS24685	128359..130476	+	2118	WP_064844259.1	carboxy terminal-processing peptidase	-
AMC90_RS24690	130634..130879	+	246	WP_064819986.1	DUF1778 domain-containing protein	-
AMC90_RS24695	130876..131370	+	495	WP_064844261.1	GNAT family N-acetyltransferase	-
AMC90_RS24700	131500..132396	+	897	WP_064844263.1	GGDEF domain-containing protein	-
AMC90_RS24705	132506..132775	-	270	WP_081284282.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..407814	407814

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14252.33 Da
• Isoelectric Point: 6.6041

>T58884 WP_064844257.1 NZ_CP013525:c127824-127441 [Rhizobium phaseoli]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRNNVIAFLAAQREALVATHDEVMMMIDRHAIFSM
GIGYTDAHLMASVLLDQRATLWTRDKRLRAAAEKAGASLHTPDNARN

Nucleotide

• Length: 384 bp

>T58884 NZ_CP013525:c127824-127441 [Rhizobium phaseoli]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCTTATGCCATCCAGCCGTGATCGGAGAGCTCGCACTTGGCAGCCTTCGGGATCGCAATAACGTGATAGCTTTCC
TGGCGGCCCAGCGCGAAGCGCTCGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGCCACGCCATTTTCAGCATG
GGCATCGGCTACACAGATGCCCATTTGATGGCTTCGGTTCTCCTCGATCAGCGGGCGACCTTATGGACGAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCCCTTCACACGCCCGACAATGCGCGAAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7154.26 Da
• Isoelectric Point: 11.3760

>AT58884 WP_016736583.1 NZ_CP013525:c128021-127821 [Rhizobium phaseoli]
MRSTINLDDTLMERARSLTGTKETATLVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT58884 NZ_CP013525:c128021-127821 [Rhizobium phaseoli]
ATGCGATCAACCATCAATCTCGACGACACCCTCATGGAAAGGGCCAGGTCCCTAACCGGCACGAAAGAAACCGCCACTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCATCGCGCTGGGCGGAACTATGCCGGATG
CGGAGGCAGCTCCACGCCGCCGGAGCGCAGCCGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W6MIA3