Detailed information of TA system TA058870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 274298..274833
• Replicon: plasmid pRspN1314d
• Accession: NZ_CP013515
• Organism: Rhizobium sp. N1314

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AMJ97_RS26545
• Protein ID: WP_064807623.1
• Coordinates: 274298..274573 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A192M8M4
• Locus tag: AMJ97_RS26550
• Protein ID: WP_064807625.1
• Coordinates: 274573..274833 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMJ97_RS26525	269507..270433	-	927	WP_064807615.1	sugar ABC transporter permease	-
AMJ97_RS26530	270486..271706	-	1221	WP_064807617.1	sugar ABC transporter substrate-binding protein	-
AMJ97_RS26535	271756..273075	-	1320	WP_064807619.1	alpha-L-fucosidase	-
AMJ97_RS26540	273284..274291	+	1008	WP_064807621.1	LacI family transcriptional regulator	-
AMJ97_RS26545	274298..274573	-	276	WP_064807623.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMJ97_RS26550	274573..274833	-	261	WP_064807625.1	ribbon-helix-helix domain-containing protein	Antitoxin
AMJ97_RS26555	275133..275861	+	729	WP_064807627.1	FadR family transcriptional regulator	-
AMJ97_RS26560	276114..277127	+	1014	WP_064807981.1	ABC transporter substrate-binding protein	-
AMJ97_RS26565	277223..278041	+	819	WP_004677388.1	ABC transporter ATP-binding protein	-
AMJ97_RS26570	278053..278934	+	882	WP_064807629.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..488795	488795

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10788.36 Da
• Isoelectric Point: 9.5543

>T58870 WP_064807623.1 NZ_CP013515:c274573-274298 [Rhizobium sp. N1314]
MEIKWTSKAVSDIARLYDFLSPVDRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
ALRLWHTREDR

Nucleotide

• Length: 276 bp

>T58870 NZ_CP013515:c274573-274298 [Rhizobium sp. N1314]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGGTCGACCGGCGAGC
TGCGGCCCGTACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCCACAATCTAT
GCATTGCGGCTTTGGCACACGCGCGAGGATCGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9670.88 Da
• Isoelectric Point: 4.6135

>AT58870 WP_064807625.1 NZ_CP013515:c274833-274573 [Rhizobium sp. N1314]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLPR

Nucleotide

• Length: 261 bp

>AT58870 NZ_CP013515:c274833-274573 [Rhizobium sp. N1314]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGATGGTGACGAG
CCGATTTCGTTGCCCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A192M8M4