Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relB-parE/ParE-RHH
Location 269156..269712 Replicon plasmid pRspN561a
Accession NZ_CP013501
Organism Rhizobium esperanzae strain N561

Toxin (Protein)


Gene name parE Uniprot ID A0A192M462
Locus tag AMJ99_RS22875 Protein ID WP_064807096.1
Coordinates 269156..269446 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A192M4C2
Locus tag AMJ99_RS22880 Protein ID WP_064807097.1
Coordinates 269434..269712 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMJ99_RS22845 264237..265004 - 768 WP_064807087.1 LamB/YcsF family protein -
AMJ99_RS22850 265026..266021 - 996 WP_064807089.1 biotin-dependent carboxyltransferase family protein -
AMJ99_RS22855 266018..266725 - 708 WP_064807091.1 5-oxoprolinase subunit PxpB -
AMJ99_RS22860 266722..267147 - 426 WP_064807198.1 hypothetical protein -
AMJ99_RS22865 267144..268538 - 1395 WP_064807093.1 acetyl-CoA carboxylase biotin carboxylase subunit -
AMJ99_RS22870 268538..268957 - 420 WP_064807094.1 acetyl-CoA carboxylase biotin carboxyl carrier protein subunit -
AMJ99_RS22875 269156..269446 - 291 WP_064807096.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMJ99_RS22880 269434..269712 - 279 WP_064807097.1 CopG family ribbon-helix-helix protein Antitoxin
AMJ99_RS22885 269835..270905 - 1071 WP_064811910.1 LacI family transcriptional regulator -
AMJ99_RS22890 271126..272076 + 951 WP_064811911.1 sugar phosphate isomerase/epimerase -
AMJ99_RS22895 272135..273367 + 1233 WP_064811912.1 ABC transporter substrate-binding protein -
AMJ99_RS22900 273470..274357 + 888 WP_064811913.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..315151 315151


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-94)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-42)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11030.78 Da        Isoelectric Point: 9.6206

>T58855 WP_064807096.1 NZ_CP013501:c269446-269156 [Rhizobium esperanzae]
MPQVIFSPAAIRDLERLREFLRPKNPSVAKRAGETILKGVSVLGAHPHIGRLIEDMPEQYREWLIDFRDSGYVARYRMDG
DILTILAVRHQKEAGF

Download         Length: 291 bp

>T58855 NZ_CP013501:c269446-269156 [Rhizobium esperanzae]
ATGCCACAAGTGATCTTCTCCCCGGCCGCAATCCGCGATCTTGAGCGGCTTCGGGAATTCTTGCGACCGAAAAACCCGTC
AGTCGCCAAGCGGGCAGGCGAAACTATTCTCAAAGGCGTGAGCGTCCTCGGTGCGCATCCCCATATAGGACGCCTCATCG
AGGATATGCCCGAGCAATACCGGGAATGGCTCATCGATTTCAGAGATAGCGGGTACGTCGCTCGTTATCGTATGGATGGC
GACATTTTGACGATCCTCGCCGTGCGGCATCAGAAGGAAGCCGGGTTCTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10667.86 Da        Isoelectric Point: 4.7901

>AT58855 WP_064807097.1 NZ_CP013501:c269712-269434 [Rhizobium esperanzae]
MASPTSLKLDDELKGRVQQLAEARRRSSHWIMREAIAQYVEREEKREALRQETLEAWNEFEATGLHVTGAEVEKWLSTWG
TDDELSAPECHK

Download         Length: 279 bp

>AT58855 NZ_CP013501:c269712-269434 [Rhizobium esperanzae]
ATGGCATCGCCAACCTCGCTCAAGCTCGATGACGAGCTGAAGGGCCGCGTCCAACAACTGGCCGAGGCGCGCCGCCGATC
GTCCCACTGGATCATGCGTGAAGCCATTGCGCAATATGTCGAGCGCGAGGAGAAGCGCGAGGCGCTGCGGCAGGAGACGC
TTGAGGCCTGGAATGAATTTGAAGCGACGGGACTGCATGTCACCGGCGCCGAGGTCGAAAAATGGCTTTCGACCTGGGGA
ACTGACGACGAGTTGTCCGCACCCGAATGCCACAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M462

Antitoxin

Source ID Structure
AlphaFold DB A0A192M4C2

References