Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 232159..232844 Replicon plasmid pRspN561a
Accession NZ_CP013501
Organism Rhizobium esperanzae strain N561

Toxin (Protein)


Gene name vapC Uniprot ID A0A192M429
Locus tag AMJ99_RS22685 Protein ID WP_064807035.1
Coordinates 232422..232844 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A192M478
Locus tag AMJ99_RS22680 Protein ID WP_064811902.1
Coordinates 232159..232425 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMJ99_RS22660 227834..228523 + 690 WP_064811899.1 ABC transporter permease -
AMJ99_RS22665 228601..229509 + 909 WP_064811900.1 homocysteine S-methyltransferase family protein -
AMJ99_RS22670 229567..230001 - 435 WP_064811901.1 MarR family transcriptional regulator -
AMJ99_RS22675 230208..232022 + 1815 WP_064807033.1 chloride channel protein -
AMJ99_RS22680 232159..232425 + 267 WP_064811902.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AMJ99_RS22685 232422..232844 + 423 WP_064807035.1 type II toxin-antitoxin system VapC family toxin Toxin
AMJ99_RS22690 232884..233654 - 771 WP_064807036.1 ABC transporter ATP-binding protein -
AMJ99_RS22695 233664..234644 - 981 WP_064807038.1 ABC transporter substrate-binding protein -
AMJ99_RS22700 234683..235534 - 852 WP_064807039.1 ABC transporter permease -
AMJ99_RS22705 235545..236897 - 1353 WP_064807041.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..315151 315151


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-60)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15569.86 Da        Isoelectric Point: 5.6376

>T58854 WP_064807035.1 NZ_CP013501:232422-232844 [Rhizobium esperanzae]
VKLLLDTNVLSEVTKPNPDVRVLEWLGKLDEDRSFISIVSVAEIRRGVALMDKGRKRDALAEWLAHDLTQRFEHRIIPVD
EPVALAWGDLMGHAKRSGRGLSSMDGLIAATAIAHDFSLATRNIRDFEGFGIELIDPWAG

Download         Length: 423 bp

>T58854 NZ_CP013501:232422-232844 [Rhizobium esperanzae]
GTGAAGCTTTTGCTGGATACCAATGTCCTGTCCGAGGTGACCAAGCCGAACCCCGACGTGCGCGTCCTTGAATGGCTGGG
CAAGCTCGACGAGGATCGTTCCTTCATCAGCATCGTGTCGGTAGCGGAGATCCGGCGCGGCGTTGCGCTGATGGACAAGG
GACGCAAACGCGACGCGCTGGCCGAATGGCTCGCCCACGACCTGACGCAACGCTTCGAGCATCGCATCATTCCGGTGGAT
GAACCGGTTGCCCTGGCCTGGGGAGACCTTATGGGCCATGCAAAACGGAGTGGCCGCGGCCTGTCGTCGATGGACGGCCT
GATCGCGGCGACAGCGATTGCTCATGATTTCAGCCTCGCGACGCGCAACATTAGGGATTTCGAGGGTTTTGGGATCGAAC
TGATCGATCCCTGGGCCGGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9828.07 Da        Isoelectric Point: 6.3426

>AT58854 WP_064811902.1 NZ_CP013501:232159-232425 [Rhizobium esperanzae]
MQPPPQADESWTVASAKAKLSEVIERAQSTPQTITRNGKPSVVVVSAEEWQRKTTRKGTLAEFLMQSPLRGADLDLERQR
DEPRDPAL

Download         Length: 267 bp

>AT58854 NZ_CP013501:232159-232425 [Rhizobium esperanzae]
ATGCAGCCGCCACCGCAAGCCGATGAAAGCTGGACCGTTGCCAGCGCAAAGGCGAAGCTTTCCGAGGTCATCGAACGCGC
GCAATCCACGCCGCAGACAATCACACGCAACGGCAAGCCCAGTGTCGTCGTCGTCTCTGCCGAGGAATGGCAGCGGAAGA
CCACCCGCAAGGGAACGCTTGCCGAATTTCTGATGCAATCGCCCTTACGCGGCGCCGATCTGGACCTTGAACGTCAACGC
GATGAACCGCGTGATCCGGCGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M429

Antitoxin

Source ID Structure
AlphaFold DB A0A192M478

References