Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 426578..427116 Replicon plasmid pRspN621d
Accession NZ_CP013499
Organism Rhizobium sp. N621

Toxin (Protein)


Gene name relE Uniprot ID A0A192M8I7
Locus tag AMK00_RS29025 Protein ID WP_064812708.1
Coordinates 426578..426856 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A192M8M4
Locus tag AMK00_RS29030 Protein ID WP_064807625.1
Coordinates 426856..427116 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK00_RS29005 421790..422716 - 927 WP_064812700.1 sugar ABC transporter permease -
AMK00_RS29010 422769..423989 - 1221 WP_064812702.1 sugar ABC transporter substrate-binding protein -
AMK00_RS29015 424039..425358 - 1320 WP_064812704.1 alpha-L-fucosidase -
AMK00_RS29020 425567..426574 + 1008 WP_064812706.1 LacI family transcriptional regulator -
AMK00_RS29025 426578..426856 - 279 WP_064812708.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AMK00_RS29030 426856..427116 - 261 WP_064807625.1 ribbon-helix-helix domain-containing protein Antitoxin
AMK00_RS29035 427416..428144 + 729 WP_064807627.1 FadR family transcriptional regulator -
AMK00_RS29040 428397..429410 + 1014 WP_064813203.1 ABC transporter substrate-binding protein -
AMK00_RS29045 429506..430324 + 819 WP_004677388.1 ABC transporter ATP-binding protein -
AMK00_RS29050 430336..431217 + 882 WP_064812710.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..742499 742499


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10875.44 Da        Isoelectric Point: 9.5543

>T58852 WP_064812708.1 NZ_CP013499:c426856-426578 [Rhizobium sp. N621]
MEIKWTSKAVSDIARLYDFLSPVDRRAAARTVQALAAAPARLIEQPRLGERLEEFDPQEVRRILVGRYEMRYEIQQSTIY
ALRLWHTREDRS

Download         Length: 279 bp

>T58852 NZ_CP013499:c426856-426578 [Rhizobium sp. N621]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGGTCGACCGGCGAGC
TGCGGCCCGTACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGAAG
AGTTCGATCCTCAGGAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GCATTGCGGCTTTGGCATACGCGCGAGGATCGGTCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9670.88 Da        Isoelectric Point: 4.6135

>AT58852 WP_064807625.1 NZ_CP013499:c427116-426856 [Rhizobium sp. N621]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLPR

Download         Length: 261 bp

>AT58852 NZ_CP013499:c427116-426856 [Rhizobium sp. N621]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAACAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGATGGTGACGAG
CCGATTTCGTTGCCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M8I7

Antitoxin

Source ID Structure
AlphaFold DB A0A192M8M4

References