Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 8018..8685 Replicon plasmid pRspN621d
Accession NZ_CP013499
Organism Rhizobium sp. N621

Toxin (Protein)


Gene name vapC Uniprot ID A0A192M7B2
Locus tag AMK00_RS27055 Protein ID WP_064812345.1
Coordinates 8018..8428 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A192M7D2
Locus tag AMK00_RS27060 Protein ID WP_064812346.1
Coordinates 8425..8685 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK00_RS27040 3565..5754 + 2190 WP_064813151.1 catalase/peroxidase HPI -
AMK00_RS27045 6072..7020 + 949 Protein_5 IS110 family transposase -
AMK00_RS27050 7182..7907 + 726 WP_064812344.1 SDR family oxidoreductase -
AMK00_RS27055 8018..8428 - 411 WP_064812345.1 type II toxin-antitoxin system VapC family toxin Toxin
AMK00_RS27060 8425..8685 - 261 WP_064812346.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AMK00_RS27065 8761..9318 - 558 WP_064812347.1 DUF3299 domain-containing protein -
AMK00_RS27070 9528..9707 - 180 WP_064812348.1 hypothetical protein -
AMK00_RS27075 9747..10322 - 576 WP_064812349.1 pyridoxamine 5'-phosphate oxidase family protein -
AMK00_RS27080 10336..11040 - 705 WP_064812350.1 SDR family oxidoreductase -
AMK00_RS27085 11147..12022 + 876 WP_064813153.1 LysR family transcriptional regulator -
AMK00_RS27090 12129..13097 - 969 WP_064813155.1 zinc-dependent alcohol dehydrogenase family protein -
AMK00_RS27095 13151..13567 + 417 WP_064812351.1 acyl-CoA thioesterase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..742499 742499


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(8-43)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14944.19 Da        Isoelectric Point: 5.6716

>T58851 WP_064812345.1 NZ_CP013499:c8428-8018 [Rhizobium sp. N621]
VITHLIDTNAVIALIGRKSDMLLARLMESDEGSIGLSTVVMHELYYGAHKSAKISYNLETLRLFMADFPAVGFEREDARA
AGEIRAALAAKGKPIGPYDVLIAAQARSRDLVLVTNNVGEFQRVEGLRVEDWTVGW

Download         Length: 411 bp

>T58851 NZ_CP013499:c8428-8018 [Rhizobium sp. N621]
GTGATTACGCATCTGATCGACACCAATGCCGTGATTGCCTTGATCGGCCGCAAGTCCGACATGCTGCTCGCTCGACTGAT
GGAGAGTGACGAGGGATCGATCGGCCTCTCCACCGTCGTCATGCACGAACTCTATTACGGGGCCCACAAGAGCGCTAAAA
TTTCCTACAATCTCGAAACCCTGCGCCTGTTCATGGCGGATTTCCCAGCCGTCGGTTTCGAGCGCGAGGATGCGCGGGCA
GCCGGGGAGATCCGCGCCGCATTGGCGGCCAAGGGAAAGCCGATCGGTCCCTACGATGTGCTGATCGCCGCGCAAGCCAG
GAGCCGCGATCTGGTTCTCGTCACCAACAATGTCGGGGAATTCCAGCGCGTCGAAGGCTTGCGCGTCGAGGATTGGACGG
TCGGGTGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9946.09 Da        Isoelectric Point: 5.1409

>AT58851 WP_064812346.1 NZ_CP013499:c8685-8425 [Rhizobium sp. N621]
MPQYARVFQSGNSQAVRLPKEFRFDVDQVEVTREGDAIILRPRTDHGVRWASLRSALERGLSDDFMAQGREQPEDQERPG
LQDLFK

Download         Length: 261 bp

>AT58851 NZ_CP013499:c8685-8425 [Rhizobium sp. N621]
ATGCCGCAATATGCGCGCGTGTTTCAATCCGGCAATTCGCAGGCCGTCCGGCTGCCGAAGGAGTTTCGCTTCGACGTGGA
TCAGGTGGAAGTGACACGGGAAGGCGATGCGATCATTCTCCGGCCGCGGACCGATCATGGCGTGCGGTGGGCCTCGTTGC
GCAGCGCCCTGGAGCGCGGCCTCAGCGACGATTTCATGGCACAAGGAAGAGAGCAACCCGAGGATCAGGAGAGGCCTGGT
CTTCAGGACCTGTTCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M7B2

Antitoxin

Source ID Structure
AlphaFold DB A0A192M7D2

References