Detailed information of TA system TA058850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 177795..178561
• Replicon: plasmid pRspN621c
• Accession: NZ_CP013498
• Organism: Rhizobium sp. N621

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A192M6G8
• Locus tag: AMK00_RS25445
• Protein ID: WP_064812117.1
• Coordinates: 178079..178561 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A192M6B1
• Locus tag: AMK00_RS25440
• Protein ID: WP_064812116.1
• Coordinates: 177795..178073 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK00_RS32265	173570..173752	-	183	WP_123876252.1	hypothetical protein	-
AMK00_RS31580	173857..174030	-	174	Protein_152	tautomerase family protein	-
AMK00_RS25415	174724..175233	+	510	WP_064808258.1	hypothetical protein	-
AMK00_RS25420	175479..175826	+	348	WP_064812112.1	helix-turn-helix domain-containing protein	-
AMK00_RS25425	175819..176322	+	504	WP_064812113.1	SRPBCC family protein	-
AMK00_RS25430	176425..176766	+	342	WP_064812114.1	hypothetical protein	-
AMK00_RS25435	176811..177644	+	834	WP_064812115.1	aminoglycoside phosphotransferase family protein	-
AMK00_RS25440	177795..178073	+	279	WP_064812116.1	DUF1778 domain-containing protein	Antitoxin
AMK00_RS25445	178079..178561	+	483	WP_064812117.1	GNAT family N-acetyltransferase	Toxin
AMK00_RS32270	178638..179048	-	411	WP_123876253.1	hypothetical protein	-
AMK00_RS25455	179244..180104	+	861	WP_064812118.1	NmrA family NAD(P)-binding protein	-
AMK00_RS25460	180219..181085	-	867	WP_064812119.1	NmrA family NAD(P)-binding protein	-
AMK00_RS25465	181204..182103	+	900	WP_064812320.1	LysR family transcriptional regulator	-
AMK00_RS25470	182274..182837	-	564	WP_064812120.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..501077	501077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-80)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17062.59 Da
• Isoelectric Point: 7.4343

>T58850 WP_064812117.1 NZ_CP013498:178079-178561 [Rhizobium sp. N621]
MLSPPALLGEAHDLQLFDSGHDGPDEWLRRRARANQVSGASRTYVVCEGERVIGYYCLSSGALAVADAPGAVRRNMPDPV
PMAVLGRLAIDLNWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFHEHYGFVASPANPMTLVLSLKARQA

Nucleotide

• Length: 483 bp

>T58850 NZ_CP013498:178079-178561 [Rhizobium sp. N621]
ATGTTGTCACCTCCGGCGCTTCTCGGAGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGATGGTCCCGATGAATG
GCTGCGCCGCCGGGCGCGGGCCAATCAGGTCAGCGGCGCGTCGCGAACCTACGTGGTCTGCGAGGGCGAGCGTGTCATCG
GTTACTATTGCCTGTCATCGGGGGCGCTCGCGGTCGCGGACGCGCCGGGAGCTGTCCGGCGCAATATGCCCGACCCCGTT
CCTATGGCAGTGCTCGGCCGTCTTGCTATCGATCTGAACTGGCAGGGAAAAGGCATTGGAGCTGCGCTGTTGCAAGACGC
CGTTTTGCGCACCGGACAGGCGGCCCATATCATGGGCATTCGTGGCCTTCTGGTTCACGCAATCTCCGACGAAGCCAAGG
CCTTTCACGAGCATTATGGGTTCGTCGCGTCGCCGGCAAACCCTATGACCTTGGTGTTATCGTTGAAAGCACGGCAGGCT
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10259.50 Da
• Isoelectric Point: 6.4818

>AT58850 WP_064812116.1 NZ_CP013498:177795-178073 [Rhizobium sp. N621]
MASKNATDTHKHAVNLKVRDDIRGLIDRAAKAHGKTRSDFMIDAARRAAEDALLDQTLVQVDPDSYEHYLAVLDQPPSGE
GFERLMNASKPW

Nucleotide

• Length: 279 bp

>AT58850 NZ_CP013498:177795-178073 [Rhizobium sp. N621]
ATGGCCAGCAAAAACGCGACCGATACCCACAAACACGCCGTCAATTTGAAGGTTCGTGACGACATTCGCGGCCTGATCGA
CCGTGCAGCCAAGGCTCACGGGAAAACCCGCTCGGATTTCATGATCGACGCCGCGCGCCGCGCCGCCGAAGACGCGCTTC
TCGATCAGACATTGGTGCAAGTCGATCCCGATAGCTATGAACACTATCTGGCCGTCCTCGACCAGCCGCCCAGCGGGGAA
GGTTTTGAGCGGCTGATGAATGCTTCCAAACCCTGGTAG

Structures

Toxin

• AlphaFold DB: A0A192M6G8

Antitoxin

• AlphaFold DB: A0A192M6B1