Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 139884..140464 Replicon plasmid pRspN621c
Accession NZ_CP013498
Organism Rhizobium sp. N621

Toxin (Protein)


Gene name vapC Uniprot ID A0A192M693
Locus tag AMK00_RS25270 Protein ID WP_064808214.1
Coordinates 139884..140267 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0T6ZM79
Locus tag AMK00_RS25275 Protein ID WP_017958375.1
Coordinates 140264..140464 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK00_RS25255 135381..136580 + 1200 WP_064808209.1 mandelate racemase/muconate lactonizing enzyme family protein -
AMK00_RS25260 136631..136930 - 300 WP_064808211.1 hypothetical protein -
AMK00_RS25265 137162..139279 + 2118 WP_064812096.1 carboxy terminal-processing peptidase -
AMK00_RS25270 139884..140267 - 384 WP_064808214.1 type II toxin-antitoxin system VapC family toxin Toxin
AMK00_RS25275 140264..140464 - 201 WP_017958375.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
AMK00_RS25280 140735..141659 + 925 Protein_126 GAF domain-containing protein -
AMK00_RS25285 142035..143003 - 969 WP_064812097.1 LysR family transcriptional regulator -
AMK00_RS25290 143105..143863 + 759 WP_064812098.1 SDR family oxidoreductase -
AMK00_RS25295 144072..144944 + 873 Protein_129 MurR/RpiR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..501077 501077


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14151.26 Da        Isoelectric Point: 6.8446

>T58849 WP_064808214.1 NZ_CP013498:c140267-139884 [Rhizobium sp. N621]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAIIGELALGSLRNRSSVIAFLAAQREALVATHDEVMMMIDRHAIFSM
GIGYTDAHLMASVLLDQRAALWTRDKRLRAAAEKAGASLHAPDNARN

Download         Length: 384 bp

>T58849 NZ_CP013498:c140267-139884 [Rhizobium sp. N621]
GTGATACTTGCCGACACTTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCTTATGCCACCCAGCCATAATCGGAGAGCTCGCACTTGGCAGCCTTCGGAATCGTAGTAGCGTCATAGCTTTCC
TGGCGGCTCAGCGCGAAGCGCTCGTTGCGACGCACGACGAAGTCATGATGATGATTGACCGCCACGCCATTTTCAGTATG
GGCATTGGCTACACAGATGCCCACTTGATGGCCTCAGTTCTCCTCGATCAACGAGCGGCCTTATGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGCGCTTCACTGCACGCCCCTGACAACGCGCGAAACTAA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7124.23 Da        Isoelectric Point: 11.3760

>AT58849 WP_017958375.1 NZ_CP013498:c140464-140264 [Rhizobium sp. N621]
MRSTINLDDTLMERARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT58849 NZ_CP013498:c140464-140264 [Rhizobium sp. N621]
ATGCGATCGACTATCAATCTCGACGACACCCTAATGGAAAGGGCCAGGTCCCTAACCGGCACGAAAGAAACCGCCGCTCT
GGTCCGCCAAGCTTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAACGCCTCATCGCTCTCGGCGGAACTATGCCGGATG
CGGAGGCAGCCCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M693

Antitoxin

Source ID Structure
AlphaFold DB A0A0T6ZM79

References