Detailed information of TA system TA058846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 143615..144171
• Replicon: chromosome
• Accession: NZ_CP013495
• Organism: Rhizobium sp. N621

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: AMK00_RS00690
• Protein ID: WP_064809224.1
• Coordinates: 143615..143779 (+)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A192LQ69
• Locus tag: AMK00_RS00695
• Protein ID: WP_064809225.1
• Coordinates: 143776..144171 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMK00_RS00660	138863..139336	-	474	WP_064800209.1	hypothetical protein	-
AMK00_RS00665	139514..140149	+	636	WP_064805816.1	trimeric intracellular cation channel family protein	-
AMK00_RS00670	140165..140584	-	420	WP_064800210.1	transcriptional repressor	-
AMK00_RS00675	140886..141401	+	516	WP_020920068.1	3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA	-
AMK00_RS00680	141447..142679	+	1233	WP_064809222.1	beta-ketoacyl-ACP synthase I	-
AMK00_RS00685	142684..143490	+	807	WP_064809223.1	enoyl-ACP reductase FabI	-
AMK00_RS00690	143615..143779	+	165	WP_064809224.1	type II toxin-antitoxin system HicA family toxin	Toxin
AMK00_RS00695	143776..144171	+	396	WP_064809225.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AMK00_RS00700	144175..145191	-	1017	WP_064809226.1	class I SAM-dependent methyltransferase	-
AMK00_RS00705	145322..147460	-	2139	WP_011423507.1	polyribonucleotide nucleotidyltransferase	-
AMK00_RS00710	147829..148098	-	270	WP_009997779.1	30S ribosomal protein S15	-
AMK00_RS00715	148248..149096	-	849	WP_064809227.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (1-48)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 5894.84 Da
• Isoelectric Point: 10.9062

>T58846 WP_064809224.1 NZ_CP013495:143615-143779 [Rhizobium sp. N621]
MKREGFEVVSIKGSHHKLRRGTETIIVPHPKKDLPTGTALAIAKQAGWSVSDKS

Nucleotide

• Length: 165 bp

>T58846 NZ_CP013495:143615-143779 [Rhizobium sp. N621]
TTGAAGCGCGAGGGCTTTGAAGTTGTTTCGATCAAAGGCTCGCACCACAAGCTTCGTAGAGGCACTGAGACCATCATCGT
TCCCCACCCGAAGAAGGATTTGCCGACCGGCACCGCGCTTGCCATTGCCAAGCAGGCCGGATGGAGTGTGAGTGACAAAT
CATGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14551.40 Da
• Isoelectric Point: 4.5667

>AT58846 WP_064809225.1 NZ_CP013495:143776-144171 [Rhizobium sp. N621]
MRYFIAIVHKDRDSAFGISFPDLPAVFSAADVEEDLTAHAIEALRLWAEDETLPMPSSYDEISSRQDIREQLTAGAFLTR
VPFIEDTTRTVRANVTFDKGMLDAIDKAAKERGLTRSAFLASAARKEIEAA

Nucleotide

• Length: 396 bp

>AT58846 NZ_CP013495:143776-144171 [Rhizobium sp. N621]
ATGAGATATTTCATCGCCATCGTGCATAAGGACAGAGACAGCGCTTTTGGTATCAGCTTTCCAGATCTGCCGGCGGTATT
CTCCGCAGCCGATGTGGAAGAAGACCTGACTGCGCATGCCATCGAAGCACTTCGCCTCTGGGCGGAGGATGAAACTCTGC
CGATGCCCTCCTCATACGACGAGATCAGCTCTCGGCAAGATATCCGCGAGCAACTGACCGCGGGGGCCTTTCTGACACGT
GTTCCCTTTATCGAAGACACGACGCGCACAGTTCGAGCCAATGTCACCTTCGACAAAGGCATGCTTGATGCGATCGACAA
GGCCGCGAAAGAACGTGGCTTGACCCGCTCGGCTTTCCTTGCAAGTGCGGCCCGCAAGGAAATCGAAGCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A192LQ69