Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 177795..178561 Replicon plasmid pRspN6212c
Accession NZ_CP013493
Organism Rhizobium sp. N6212

Toxin (Protein)


Gene name tacT Uniprot ID A0A192M6G8
Locus tag AMK01_RS25455 Protein ID WP_064812117.1
Coordinates 178079..178561 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A192M6B1
Locus tag AMK01_RS25450 Protein ID WP_064812116.1
Coordinates 177795..178073 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK01_RS32270 173570..173752 - 183 WP_123876252.1 hypothetical protein -
AMK01_RS31570 173857..174030 - 174 Protein_152 tautomerase family protein -
AMK01_RS25425 174724..175233 + 510 WP_064808258.1 hypothetical protein -
AMK01_RS25430 175479..175826 + 348 WP_064812112.1 helix-turn-helix domain-containing protein -
AMK01_RS25435 175819..176322 + 504 WP_064812113.1 SRPBCC family protein -
AMK01_RS25440 176425..176766 + 342 WP_064812114.1 hypothetical protein -
AMK01_RS25445 176811..177644 + 834 WP_064812115.1 aminoglycoside phosphotransferase family protein -
AMK01_RS25450 177795..178073 + 279 WP_064812116.1 DUF1778 domain-containing protein Antitoxin
AMK01_RS25455 178079..178561 + 483 WP_064812117.1 GNAT family N-acetyltransferase Toxin
AMK01_RS32275 178638..179048 - 411 WP_123876253.1 hypothetical protein -
AMK01_RS25465 179244..180104 + 861 WP_064812118.1 NmrA family NAD(P)-binding protein -
AMK01_RS25470 180219..181085 - 867 WP_064812119.1 NmrA family NAD(P)-binding protein -
AMK01_RS25475 181204..182103 + 900 WP_064812320.1 LysR family transcriptional regulator -
AMK01_RS25480 182274..182837 - 564 WP_064812120.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..501077 501077


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-142)

Antitoxin

Vibrio phage ICP1, Orf50

(1-80)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17062.59 Da        Isoelectric Point: 7.4343

>T58843 WP_064812117.1 NZ_CP013493:178079-178561 [Rhizobium sp. N6212]
MLSPPALLGEAHDLQLFDSGHDGPDEWLRRRARANQVSGASRTYVVCEGERVIGYYCLSSGALAVADAPGAVRRNMPDPV
PMAVLGRLAIDLNWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFHEHYGFVASPANPMTLVLSLKARQA

Download         Length: 483 bp

>T58843 NZ_CP013493:178079-178561 [Rhizobium sp. N6212]
ATGTTGTCACCTCCGGCGCTTCTCGGAGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGATGGTCCCGATGAATG
GCTGCGCCGCCGGGCGCGGGCCAATCAGGTCAGCGGCGCGTCGCGAACCTACGTGGTCTGCGAGGGCGAGCGTGTCATCG
GTTACTATTGCCTGTCATCGGGGGCGCTCGCGGTCGCGGACGCGCCGGGAGCTGTCCGGCGCAATATGCCCGACCCCGTT
CCTATGGCAGTGCTCGGCCGTCTTGCTATCGATCTGAACTGGCAGGGAAAAGGCATTGGAGCTGCGCTGTTGCAAGACGC
CGTTTTGCGCACCGGACAGGCGGCCCATATCATGGGCATTCGTGGCCTTCTGGTTCACGCAATCTCCGACGAAGCCAAGG
CCTTTCACGAGCATTATGGGTTCGTCGCGTCGCCGGCAAACCCTATGACCTTGGTGTTATCGTTGAAAGCACGGCAGGCT
TGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10259.50 Da        Isoelectric Point: 6.4818

>AT58843 WP_064812116.1 NZ_CP013493:177795-178073 [Rhizobium sp. N6212]
MASKNATDTHKHAVNLKVRDDIRGLIDRAAKAHGKTRSDFMIDAARRAAEDALLDQTLVQVDPDSYEHYLAVLDQPPSGE
GFERLMNASKPW

Download         Length: 279 bp

>AT58843 NZ_CP013493:177795-178073 [Rhizobium sp. N6212]
ATGGCCAGCAAAAACGCGACCGATACCCACAAACACGCCGTCAATTTGAAGGTTCGTGACGACATTCGCGGCCTGATCGA
CCGTGCAGCCAAGGCTCACGGGAAAACCCGCTCGGATTTCATGATCGACGCCGCGCGCCGCGCCGCCGAAGACGCGCTTC
TCGATCAGACATTGGTGCAAGTCGATCCCGATAGCTATGAACACTATCTGGCCGTCCTCGACCAGCCGCCCAGCGGGGAA
GGTTTTGAGCGGCTGATGAATGCTTCCAAACCCTGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M6G8

Antitoxin

Source ID Structure
AlphaFold DB A0A192M6B1

References