Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 232162..232847 Replicon plasmid pRspN6212a
Accession NZ_CP013491
Organism Rhizobium sp. N6212

Toxin (Protein)


Gene name vapC Uniprot ID A0A192M429
Locus tag AMK01_RS22290 Protein ID WP_064807035.1
Coordinates 232425..232847 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A192M478
Locus tag AMK01_RS22285 Protein ID WP_064811902.1
Coordinates 232162..232428 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AMK01_RS22265 227837..228526 + 690 WP_064811899.1 ABC transporter permease -
AMK01_RS22270 228604..229512 + 909 WP_064811900.1 homocysteine S-methyltransferase family protein -
AMK01_RS22275 229570..230004 - 435 WP_064811901.1 MarR family transcriptional regulator -
AMK01_RS22280 230211..232025 + 1815 WP_064807033.1 chloride channel protein -
AMK01_RS22285 232162..232428 + 267 WP_064811902.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AMK01_RS22290 232425..232847 + 423 WP_064807035.1 type II toxin-antitoxin system VapC family toxin Toxin
AMK01_RS22295 232887..233657 - 771 WP_064807036.1 ABC transporter ATP-binding protein -
AMK01_RS22300 233667..234647 - 981 WP_064807038.1 ABC transporter substrate-binding protein -
AMK01_RS22305 234686..235537 - 852 WP_064807039.1 ABC transporter permease -
AMK01_RS22310 235548..236900 - 1353 WP_064807041.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..315154 315154


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-60)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15569.86 Da        Isoelectric Point: 5.6376

>T58840 WP_064807035.1 NZ_CP013491:232425-232847 [Rhizobium sp. N6212]
VKLLLDTNVLSEVTKPNPDVRVLEWLGKLDEDRSFISIVSVAEIRRGVALMDKGRKRDALAEWLAHDLTQRFEHRIIPVD
EPVALAWGDLMGHAKRSGRGLSSMDGLIAATAIAHDFSLATRNIRDFEGFGIELIDPWAG

Download         Length: 423 bp

>T58840 NZ_CP013491:232425-232847 [Rhizobium sp. N6212]
GTGAAGCTTTTGCTGGATACCAATGTCCTGTCCGAGGTGACCAAGCCGAACCCCGACGTGCGCGTCCTTGAATGGCTGGG
CAAGCTCGACGAGGATCGTTCCTTCATCAGCATCGTGTCGGTAGCGGAGATCCGGCGCGGCGTTGCGCTGATGGACAAGG
GACGCAAACGCGACGCGCTGGCCGAATGGCTCGCCCACGACCTGACGCAACGCTTCGAGCATCGCATCATTCCGGTGGAT
GAACCGGTTGCCCTGGCCTGGGGAGACCTTATGGGCCATGCAAAACGGAGTGGCCGCGGCCTGTCGTCGATGGACGGCCT
GATCGCGGCGACAGCGATTGCTCATGATTTCAGCCTCGCGACGCGCAACATTAGGGATTTCGAGGGTTTTGGGATCGAAC
TGATCGATCCCTGGGCCGGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9828.07 Da        Isoelectric Point: 6.3426

>AT58840 WP_064811902.1 NZ_CP013491:232162-232428 [Rhizobium sp. N6212]
MQPPPQADESWTVASAKAKLSEVIERAQSTPQTITRNGKPSVVVVSAEEWQRKTTRKGTLAEFLMQSPLRGADLDLERQR
DEPRDPAL

Download         Length: 267 bp

>AT58840 NZ_CP013491:232162-232428 [Rhizobium sp. N6212]
ATGCAGCCGCCACCGCAAGCCGATGAAAGCTGGACCGTTGCCAGCGCAAAGGCGAAGCTTTCCGAGGTCATCGAACGCGC
GCAATCCACGCCGCAGACAATCACACGCAACGGCAAGCCCAGTGTCGTCGTCGTCTCTGCCGAGGAATGGCAGCGGAAGA
CCACCCGCAAGGGAACGCTTGCCGAATTTCTGATGCAATCGCCCTTACGCGGCGCCGATCTGGACCTTGAACGTCAACGC
GATGAACCGCGTGATCCGGCGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A192M429

Antitoxin

Source ID Structure
AlphaFold DB A0A192M478

References