Detailed information of TA system TA058831

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4127000..4127835
• Replicon: chromosome
• Accession: NZ_CP013483
• Organism: Escherichia coli strain Y5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: ATO45_RS20360
• Protein ID: WP_042047257.1
• Coordinates: 4127000..4127377 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: ATO45_RS20365
• Protein ID: WP_001295723.1
• Coordinates: 4127467..4127835 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATO45_RS25955	4123185..4123358	+	174	Protein_3850	DUF4102 domain-containing protein	-
ATO45_RS20335	4123373..4124070	-	698	Protein_3851	IS1 family transposase	-
ATO45_RS20340	4124126..4125280	+	1155	Protein_3852	site-specific integrase	-
ATO45_RS20345	4125373..4126221	-	849	WP_042047262.1	DUF4942 domain-containing protein	-
ATO45_RS20350	4126306..4126503	-	198	WP_000839267.1	DUF957 domain-containing protein	-
ATO45_RS20355	4126515..4127003	-	489	WP_042047259.1	hypothetical protein	-
ATO45_RS20360	4127000..4127377	-	378	WP_042047257.1	TA system toxin CbtA family protein	Toxin
ATO45_RS20365	4127467..4127835	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ATO45_RS20375	4127998..4128219	-	222	WP_000692311.1	DUF987 domain-containing protein	-
ATO45_RS20380	4128282..4128758	-	477	WP_001186774.1	RadC family protein	-
ATO45_RS20385	4128774..4129259	-	486	WP_000849588.1	antirestriction protein	-
ATO45_RS20390	4129314..4130132	-	819	WP_001234620.1	DUF945 domain-containing protein	-
ATO45_RS20395	4130232..4130465	-	234	WP_001119729.1	DUF905 family protein	-
ATO45_RS20400	4130544..4130999	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14211.22 Da
• Isoelectric Point: 7.2652

>T58831 WP_042047257.1 NZ_CP013483:c4127377-4127000 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T58831 NZ_CP013483:c4127377-4127000 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT58831 WP_001295723.1 NZ_CP013483:c4127835-4127467 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT58831 NZ_CP013483:c4127835-4127467 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1NM52