Detailed information of TA system TA058829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2873465..2874103
• Replicon: chromosome
• Accession: NZ_CP013483
• Organism: Escherichia coli strain Y5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: ATO45_RS14110
• Protein ID: WP_001447010.1
• Coordinates: 2873927..2874103 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ATO45_RS14105
• Protein ID: WP_001270286.1
• Coordinates: 2873465..2873881 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ATO45_RS14085	2868617..2869558	-	942	WP_001251304.1	ABC transporter permease	-
ATO45_RS14090	2869559..2870572	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
ATO45_RS14095	2870590..2871735	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
ATO45_RS14100	2871980..2873386	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
ATO45_RS14105	2873465..2873881	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
ATO45_RS14110	2873927..2874103	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
ATO45_RS14115	2874325..2874555	+	231	WP_000494244.1	YncJ family protein	-
ATO45_RS14120	2874647..2876608	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
ATO45_RS14125	2876681..2877217	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
ATO45_RS14130	2877270..2878484	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T58829 WP_001447010.1 NZ_CP013483:c2874103-2873927 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T58829 NZ_CP013483:c2874103-2873927 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT58829 WP_001270286.1 NZ_CP013483:c2873881-2873465 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT58829 NZ_CP013483:c2873881-2873465 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8