Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1346574..1347228 Replicon chromosome
Accession NZ_CP013483
Organism Escherichia coli strain Y5

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ATO45_RS06760 Protein ID WP_000244777.1
Coordinates 1346821..1347228 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ATO45_RS06755 Protein ID WP_000354046.1
Coordinates 1346574..1346840 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATO45_RS06730 1341743..1342486 + 744 WP_000951964.1 SDR family oxidoreductase -
ATO45_RS06735 1342543..1343976 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ATO45_RS06740 1344021..1344332 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ATO45_RS06745 1344496..1345155 + 660 WP_000250274.1 hemolysin III family protein -
ATO45_RS06750 1345351..1346331 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ATO45_RS06755 1346574..1346840 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ATO45_RS06760 1346821..1347228 + 408 WP_000244777.1 toxin CptA Toxin
ATO45_RS06765 1347268..1347789 - 522 WP_001055874.1 flavodoxin FldB -
ATO45_RS06770 1347901..1348797 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ATO45_RS06775 1348822..1349532 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ATO45_RS06780 1349538..1351271 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T58817 WP_000244777.1 NZ_CP013483:1346821-1347228 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T58817 NZ_CP013483:1346821-1347228 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT58817 WP_000354046.1 NZ_CP013483:1346574-1346840 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT58817 NZ_CP013483:1346574-1346840 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References