Detailed information of TA system TA058811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2042437..2043095
• Replicon: chromosome
• Accession: NZ_CP013481
• Organism: Pandoraea apista strain DSM 16535

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5E5PAN6
• Locus tag: AT395_RS09470
• Protein ID: WP_048629124.1
• Coordinates: 2042916..2043095 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A5E5P9T0
• Locus tag: AT395_RS09465
• Protein ID: WP_048629123.1
• Coordinates: 2042437..2042841 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AT395_RS09415	2037836..2038411	+	576	WP_058375181.1	DUF4376 domain-containing protein	-
AT395_RS09420	2038434..2038970	+	537	WP_048629114.1	glycoside hydrolase family 104 protein	-
AT395_RS09425	2038985..2039278	+	294	WP_048629115.1	holin	-
AT395_RS09430	2039334..2039849	+	516	WP_048629116.1	hypothetical protein	-
AT395_RS09435	2039846..2040040	+	195	WP_048629117.1	hypothetical protein	-
AT395_RS09440	2040109..2040351	+	243	WP_124988637.1	hypothetical protein	-
AT395_RS09445	2040362..2040787	+	426	WP_048629119.1	hypothetical protein	-
AT395_RS09450	2040784..2041146	+	363	WP_048629120.1	DUF1353 domain-containing protein	-
AT395_RS09455	2041246..2042001	-	756	WP_048629121.1	SOS response-associated peptidase family protein	-
AT395_RS09465	2042437..2042841	-	405	WP_048629123.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AT395_RS09470	2042916..2043095	-	180	WP_048629124.1	type II toxin-antitoxin system HicA family toxin	Toxin
AT395_RS09475	2043209..2043838	+	630	WP_048629125.1	SOS response-associated peptidase family protein	-
AT395_RS09485	2044153..2045268	-	1116	WP_042115266.1	YeeE/YedE family protein	-
AT395_RS09490	2045600..2046319	-	720	WP_094068917.1	tol-pal system protein YbgF	-
AT395_RS09495	2046354..2046854	-	501	WP_042115270.1	OmpA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1973710..2043838	70128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6546.79 Da
• Isoelectric Point: 11.0075

>T58811 WP_048629124.1 NZ_CP013481:c2043095-2042916 [Pandoraea apista]
MNSSKIIRMLEDDGWKLARITGSHHHFKHPTKPLLVTVPHPKKDLPVGTVKSILKAAGL

Nucleotide

• Length: 180 bp

>T58811 NZ_CP013481:c2043095-2042916 [Pandoraea apista]
ATGAATTCATCGAAAATCATCCGGATGCTCGAAGATGATGGTTGGAAGTTAGCGCGAATCACTGGCAGCCACCACCACTT
TAAACACCCAACGAAGCCGCTACTTGTGACGGTGCCTCACCCGAAGAAGGATCTGCCAGTTGGGACTGTTAAAAGCATCC
TGAAAGCCGCCGGCCTGTAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14923.74 Da
• Isoelectric Point: 4.5412

>AT58811 WP_048629123.1 NZ_CP013481:c2042841-2042437 [Pandoraea apista]
MEFPIAIHKDDGSVYGVTVPDVPGCFSWGETIDDAIRNAKEAIKGHIETSLEYSEDVTFSCSDIEDLAKREEFAGAVWAL
ADVDVTKLDSKPERINVSFPRFVLHKIDAYVEKRHETRSGFLARVALEALAHEA

Nucleotide

• Length: 405 bp

>AT58811 NZ_CP013481:c2042841-2042437 [Pandoraea apista]
ATGGAATTTCCCATCGCAATTCATAAGGATGATGGGAGTGTCTATGGAGTGACAGTGCCGGATGTTCCGGGCTGCTTCTC
CTGGGGCGAAACCATCGACGACGCCATCAGGAATGCAAAGGAAGCAATCAAAGGCCATATCGAAACGTCGCTTGAGTATT
CGGAAGATGTGACGTTTTCCTGCTCAGACATCGAGGATTTGGCTAAGCGTGAGGAATTTGCTGGCGCCGTTTGGGCTTTA
GCAGATGTCGATGTAACAAAGCTTGATAGCAAACCGGAGCGCATCAACGTTAGCTTCCCGCGCTTCGTCCTTCACAAGAT
CGATGCTTACGTTGAAAAACGCCATGAGACCCGTAGCGGCTTTCTCGCTCGCGTCGCTTTGGAGGCGCTTGCCCATGAAG
CTTGA

Structures

Toxin

• AlphaFold DB: A0A5E5PAN6

Antitoxin

• AlphaFold DB: A0A5E5P9T0